HPLC测定藏药风毛菊中茵芋苷、东莨菪苷和伞形花内酯的含量  

Determination of skimmin,scopolin and umbelliferone in Tibetan medicine Saussurea hieracioides by HPLC

作　　者：谭荣[1] 王毓杰[1] 张雨欣[1] 周礼仕 谭尔[1] 阿萍 张艺[1] 

机构地区：[1]成都中医药大学民族医药学院,四川成都611137 [2]西藏自治区食品药品检验所,西藏拉萨850000

出　　处：《中国中药杂志》2014年第6期1054-1057,共4页China Journal of Chinese Materia Medica

基　　金：国家药典委员会国家药品标准提高项目(896);四川省教育厅创新团队项目(11TD004)

摘　　要：该文建立了HPLC同时测定藏药风毛菊中茵芋苷、东莨菪苷和伞形花内酯3种香豆素类成分含量的方法。采用Wondasil C18-WR色谱柱（4.6 mm×250 mm，5 μm），以甲醇（A）-0.1%磷酸水溶液（B）为流动相，梯度洗脱，流速1.0 mL·min^-1；检测波长325 nm；进样量10 μL；柱温35 ℃。结果茵芋苷，东莨菪苷和伞形花内酯在40 min内均达到基线分离，各成分在其线性范围内均呈现良好的线性关系，线性范围分别为0.18-5.6 μg（r=1.000 0），0.060-1.8 μg（r=0.999 9），0.032-0.97 μg（r=0.999 8）。平均加样回收率分别为99.16%（RSD 0.41%），100.3%（RSD 0.79%），102.2%（RSD 0.87%）。该方法操作简便，准确，重复性和稳定性较好，可作为藏药风毛菊的质量评价提供科学依据。This study is aimed to establish a high-performance liquid chromatography （HPLC） method for simultaneous determination of skimmin, scopolin and umbelliferone in Saussurea hieracioides. Samples were analyzed on a Wondasil C18-WR column （4.6 mm×250 mm, 5 μm） with methanol （A） and water containing 0.1% phosphate （B） as mobile phases for gradient elution at a flow rate of 1.0 mL·min^-1. The detection wavelength and column temperature were set at 325 nm and 35 ℃, respectively, and the sample size was 10 μL. The results showed that skimmin, scopolin and umbelliferone were simultaneously achieved within 40 min under the above conditions. A good linearity was observed in the range of 0.18-5.6 μg （r=1.000 0）, 0.060-1.8 μg （r=0.999 9）, 0.032-0.97 μg （r=0.999 8） for skimmin, scopolin and umbelliferone, respectively, with the average recoveries of 99.16% （RSD=0.41% ）, 100.3% （RSD=0.79%）, 102.2% （RSD=0.87%）. The method is simple, accurate and reproducible and can be used for the quality control of S. hieracioides.

关 键 词：藏药 风毛菊 HPLC 含量测定 质量控制 

分 类 号：R284.1[医药卫生—中药学]