丹参酮Ⅱ_A磺酸钠对AngⅡ诱导心房成纤维细胞胶原合成及TGF-β_1活化的影响  

Effect of sodium tanshinone Ⅱ_A sulfonate on AngⅡ-induced atrial fibroblast collagen synthesis and TGF-β_1 activation

作　　者：杨乐[1] 邹晓静[2] 尹钊[3] 郝洪真 

机构地区：[1]华中科技大学同济医院急诊内科,湖北武汉430030 [2]华中科技大学协和医院麻醉科,湖北武汉430022 [3]华中科技大学同济医学院药理学系,湖北武汉430030

出　　处：《中国中药杂志》2014年第6期1093-1096,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81102691,30901405);湖北省自然科学基金项目(2011CDB538)

摘　　要：目的:观察丹参酮ⅡA磺酸钠(sodium tanshinoneⅡAsulfonate,STS)对血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)诱导心房成纤维细胞胶原合成及TGF-β1活化的影响。方法:培养新生大鼠心房成纤维细胞,检测胶原含量,并[3H]-脯氨酸掺入法测定胶原合成速率作为心肌纤维化指标。ELISA法检测培养细胞上清中活性TGF-β1及总TGF-β1含量。Western blot测定血小板反应素-1(thrombospondin-1,TSP-1)表达。结果:AngⅡ能够明显上调心房成纤维细胞胶原含量、胶原合成速率;TSP-1表达及活性TGF-β1分泌量上调,而总TGF-β1表达无明显改变。在STS处理后,除总TGF-β1外,其他指标均明显下调。结论:STS能减轻AngⅡ诱导心房成纤维细胞胶原分泌及合成速率,机制与抑制TSP-1/TGF-β1通路有关。Objective： To observe the effect of sodium tanshinone ⅡA sulfonate （STS） on Ang Ⅱ-induced atrial fibroblast collagen synthesis and TGF-β1 activation. Method： Atrial fibroblasts of neonatal rats were cultured to determine the content of collagen protein. The original synthesis rate determined by the [3H]-proline incorporation method was taken as the index for myocardial fibrosis. The content of active TGF-β1 and total TGF-β1 in cell culture supernatants were tested and cultured by ELISA. The expression of thrombospondin-1 （TSP-1） was assessed by using Western blot. Result： Ang Ⅱ could significantly increase the content of atrial fibroblast collagen and the collagen synthesis rate, the TSP-1 expression and the concentration of active TGF-β1, without any obvious change in total TGF-β1. After the STS treatment, all of the indexes, apart from total TGF-β1, were obviously down-regulated. Conclusion： STS could decrease the secretion of Ang Ⅱ-induced atrial fibroblast collagen and the synthesis rate. Its mechanism is related to the inhibition of TSP-1/TGF-β1 pathway.

关 键 词：丹参酮ⅡA磺酸钠 心房纤维化 转化生长因子-Β1 

分 类 号：R541.75[医药卫生—心血管疾病]