红景天提高黑腹果蝇肠道免疫功能研究  

Emprovement of Rhodiola rosea on intestinal immune function of Drosophila melanogaster

作　　者：左占广 朱彩霞[1] 鲁东立 金丽华[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《中草药》2014年第5期691-694,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31070775;31270923);国家基础科学人才培养基金-科研训练及科研能力提高项目资助(J1210053);东北林业大学本科创新项目(201210225109)

摘　　要：目的研究红景天提取物对果蝇肠道免疫功能的影响。方法利用真菌和十二烷基硫酸钠(SDS)诱导果蝇肠道损伤,通过比较果蝇生存率、肠道上皮细胞死亡、肠道上皮细胞内活性氧(ROS)的量和肠道形态变化等,探讨红景天提取物对果蝇肠道免疫功能的影响。结果红景天提取物可明显提高Beauveria bassiana孢子和SDS诱导后果蝇的生存率(P<0.05);降低肠道上皮细胞的死亡和肠道上皮细胞内ROS的量;保护和维持肠道形态。结论红景天提取物对真菌和SDS诱导的肠道免疫损伤具有很好的缓解和保护作用。Objective To study the effect of Rhodiola rosea extracts on intestinal immune fimction of Drosophila melanogaster. Methods D. melanogaster was treated by SDS and fungal biological solution with or without R. rosea extracts, and the effect of R. rosea extracts on the survival rate of D. melanogaster, intestinal epithelium cell death, relative contents of reactive oxygen species （ROS） in intestinal epithelium cells, and intestinal morphology changes were analyzed. Results R. rosea extracts could significantly improve the survival rate of SDS and fungus （Beauveria bassiana） -infected D. melanogaster （P 〈 0.05）, reduce the intestinal epithelial cell death and the level of intestinal ROS levels, and protect and maintain the intestinal morphology. Conclusion R. rosea extracts could significantly improve the intestinal immune function of D. melanogaster with the amelioration and protection of intestinal immunologic function injury induced by fungi and SDS.

关 键 词：红景天 黑腹果蝇 肠道免疫 肠道上皮细胞 生存率 

分 类 号：R285.5[医药卫生—中药学]