一氧化氮和水杨酸依次介导内生真菌诱导子促进苍术细胞中苍术素生物合成的信号转导  

Signal transduction of atractylodin biosynthesis in Atractylodes lancea cell induced by endophytic fungal elicitor mediated with nitric oxide followed by salicylic acid

作　　者：陶金华[1] 汪冬庚[1] 濮雪莲[1] 黄金华[1] 赵喜[1] 仇雯倩[1] 江曙[2] 

机构地区：[1]南通大学,江苏南通226019 [2]南京中医药大学,江苏南京210023

出　　处：《中草药》2014年第5期701-708,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81102743)

摘　　要：目的研究介导内生真菌诱导子促进苍术悬浮细胞中苍术素生物合成的信号分子及信号转导途径,并探讨诱导子对苍术素合成途径关键酶活性的影响。方法采用植物细胞悬浮培养法,考察内生真菌诱导子处理下苍术细胞中一氧化氮(NO)、水杨酸(SA)及苍术素量的变化。结果内生真菌诱导子通过诱导苍术细胞中一氧化氮合酶(NOS)、苯丙氨酸解氨酶(PAL)以及乙酰辅酶A羧化酶(ACC)的活性,显著促进苍术细胞的NO迸发、SA和苍术素的合成。NOS抑制剂PBITU可以阻断诱导子对NO、SA和苍术素合成的促进作用,外源NO供体SNP及外源SA单独处理也能促进苍术素的合成,说明NO和SA是参与苍术素合成的信号分子,且NOS是参与诱导子诱发苍术细胞NO迸发的主要途径。NO猝灭剂cPITO可以有效清除诱导子诱发苍术细胞的NO迸发,显著阻断诱导子对苍术细胞中SA和苍术素合成的促进作用,外源SNP可以逆转cPITO对PAL、ACC活性以及SA、苍术素合成的抑制作用,表明NO是介导内生真菌诱导子诱发苍术细胞中苍术素和SA生物合成所必需的上游信号分子。结论NO主要通过SA信号途径介导内生真菌诱导子激活ACC,显著促进苍术细胞中苍术素的生物合成。Objective To investigate the signal molecules and signal transduction involved in endophytic fungal elicitor-induced atractylodin biosynthesis and the effect of an endophytic fi.mgal elicitor on the key enzyme activity in Atractylodes lancea. Methods Content changes of nitric oxide （NO）, salicylic acid （SA）, and atractylodin were detected under the endophytic fungal elicitor treatment by plant cell suspension culture technology. Results The endophytic fungal elicitor remarkably promoted NO burst and the biosynthesis of SA and atractyodin by activating nitric oxide synthase （NOS）, phenylalanine ammonia lyase （PAL）, and acetyl coenzyme A carboxylase （ACC）, respectively. NOS inhibitor PBITU could inhibit the NO and SA accumulation and the atractyodin biosynthesis induced by the elicitor. And atractyodin biosynthesis could also be triggered by exogenous NO or SA. The results indicated that NO and SA were the necessary signal molecules and NO burst was mediated by NOS induced by endophytic fungal elicitor. NO quencher cPITO could effectively remove NO burst in A. lancea cell induced by endophytic fungal elicitor and notably inhibit the biosynthesis promotion of SA and atractyodin in A. lancea cell induced by endophytic fungal elicitor. Exogenous SNP could reverse the cPITO inhibition on the activity of PAL and ACC and the synthesis of SA and atractylodin. This suggested that NO was an upstream signal molecule mediated endophytic ftmgal elicitor to accelerate the biosynthesis of SA and atractyodin. Conclusion Endophytic fi.mgal elicitor mediated through NO followed by SA could promote atractyodin biosynthesis by activating ACC in A. lancea.

关 键 词：内生真菌诱导子 苍术 一氧化氮 水杨酸 苍术素 一氧化氮合酶 苯丙氨酸解氨酶 乙酰辅酶A羧化酶 

分 类 号：R282.13[医药卫生—中药学]