基于修饰石墨烯/纳米金复合膜固定乙酰胆碱酯酶的敌百虫传感器  

A Novel Trichlorfon Biosensor Based on Acetylcholinesterase Immobilized by Modified Graphene/nano-Au Composite

作　　者：展海军[1] 李洁[1] 郑莹莹[1] 刘志敏[1] 

机构地区：[1]河南工业大学化学化工学院,河南郑州450001

出　　处：《分析测试学报》2014年第3期277-282,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(21275039);河南省高校青年骨干教师基金资助项目(001135)

摘　　要：结合氨基功能化离子液体修饰石墨烯（IL-GR）、纳米金（Au）等纳米材料的独特性质,以壳聚糖（CHIT）为交联剂,首先在玻碳电极表面固定IL-GR,然后吸附胶体金制得Au/IL-GR-CHIT复合膜,最后固定乙酰胆碱酯酶（AChE）制得新型有机磷检测酶传感器（AChE/Au/IL-GR-CHIT/GCE）,并用于白菜样品中敌百虫农药的测定.采用透射电镜（TEM）对纳米材料进行了表征,循环伏安法（CV）和差示脉冲伏安法（DPV）研究了传感器的电化学性质.纳米复合物不仅为保持AChE的生物活性提供了适宜的微环境,并且对传感器性能的改善显示出强大的协同效应.在优化实验条件下,抑制率（A）与敌百虫浓度的负对数在2.0×10-10～ 1.0×10-6mol/L范围内呈良好的线性关系,检出限（S/N=3）为2.1＆#215;10-12 mol/L.用于蔬菜中敌百虫含量的测定,回收率为97.5％～107.2％.According to the special properties of functionalized ionic liquid modified graphene （IL- GR） and nano-Au, IL- GR was firstly immobilized on the glass carbon electrode via chitosan （CHIT） cross-linking, and then a layer of nano-Au was adsorbed to form Au/IL - GR - CHIT com- posite film. Finally, acetylcholinesterase（ACHE） was immobilized on the composite film by adsorp- tion. A novel biosensor for detection of organophosphorus pesticides enzyme was developed, and used for the determination of trichlorfon. Different technologies were employed to study the construc- tion process and the electrochemical properties of the biosensor. The results showed that the nano- composite not only provided a biocompatible microenvironment to keep the bioactivity of ACHE, but also exhibited a strong synergetic effect improving the sensing properties of biosensor. Linear relation- ship between inhibition percentage （A） and logarithm of concentration of trichlorfon was found in the range of 2.0 × 10- l0 - 1.0× 10-6 mol/L, and the detection limit（ S/N= 3 ） was calculated to be 2. 1 × 10 -12 mol/L. The recoveries of trichlorfon in vegetable ranged from 97.5% to 107.2% .

关 键 词：石墨烯 离子液体 纳米金 乙酰胆碱酯酶生物传感器 敌百虫 

分 类 号：O657.1[理学—分析化学] F767.2[理学—化学]