盐生盐杆菌含耐盐相关基因DNA片段的克隆  

Cloning of Halo-tolerant-Related Gene(s) from Halobacterium halobium

作　　者：谭静[1] 刘广发[1] 

机构地区：[1]厦门大学生命科学学院

出　　处：《厦门大学学报（自然科学版）》2001年第1期146-149,共4页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金!资助项目 ( C960 0 4 )

摘　　要：将盐生盐杆菌总 DNA的 Bam HI不完全酶切片段与质粒 p U C19连接成重组质粒 ,并以重组质粒转化 E. coli JM10 1.经 X- Gal- IPTG法筛选白色重组子 ,结合高盐平板检测 ,已经获得 3株比原受体菌的耐盐性提高 30 %～ 67%的转化子 .重组质粒酶切电泳分析表明 ,被克隆的盐生盐杆菌含耐盐相关基因的 DNA片段长度在 1.0～ 3.5kb之间 .Three recombinant plasmids which have been reconstructed with plasmid pUC19 and random DNA fragments of Halobacterium halobium were transferred to E. coli JM101. As the result, the tolerant level against to NaCl of these bacteria transferred has been raised from 0.9 to 1.2~1.5 mol·L -1 respectively. The molecular weight of these DNA fragments inserted are 1.0 , 1.8 and 3.5 kb according to the evidents of agarose eletrophoresis. So it could be referred that these three DNA fragements may contain halo tolerant related gene(s).

关 键 词：盐生盐杆菌 耐盐基因 克隆 盐碱地改良 

分 类 号：Q933[生物学—微生物学] S156.4[农业科学—土壤学]