β3肾上腺素能受体基因多态性与糖尿病足的相关性研究  

Relationship between gene polymorphism of beta 3-adrenergic receptor and diabetic foot

作　　者：惠宗光[1] 周学伟[2] 李文通[3] 孙金红[1] 侯宁宁[1] 孙红喜[1] 

机构地区：[1]潍坊医学院附属医院内分泌科,261041 [2]潍坊医学院附属医院药剂科,261041 [3]潍坊医学院病理学教研室,261042

出　　处：《国际内分泌代谢杂志》2014年第2期138-140,共3页International Journal of Endocrinology and Metabolism

基　　金：山东省高等学校科技计划基金项目（J12LK05）

摘　　要：目的 探讨中国人群β3肾上腺素能受体（β3-AR）基因Trp64Arg突变与糖尿病足的关系.方法 应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测110例2型糖尿病患者（T2DM组）[其中糖尿病足患者58例（DFU组）,单纯2型糖尿病患者52例（N-DFU组）]和42例正常人（正常对照组）β3-AR基因的Trp64Arg突变,比较各组的基因型（TT型,TA型或AA型）和基因频率（T或A）；并进行人体测量学和代谢指标的检测.结果 （1）β3-AR基因Trp64Arg基因型频率符合Hardy-Weinberg平衡定律,T2DM组β-AR基因64位Arg突变患者,其体重指数和稳态模型评估-胰岛素抵抗指数（HOMA-IR）显著高于无突变组（P=0.007 2,P=0.038 6）.（2）在T2DM组,Arg64携带者血高密度脂蛋白-胆固醇水平明显低于非携带者（P =0.047 5）.（3）DFU组Arg64等位基因频率明显高于N-DFU组（x2=5.147,P＜0.05）,而T2DM组与正常对照组β3-AR基因型及等位基因频率无统计学意义（x2=0.067,P ＞0.05）.结论 β3-AR基因多态性与糖尿病足存在相关性,Ar64等位基因可能是糖尿病足的危险因素之一.Objective To explore the relationship between Trp64Arg polymorphism of the β3-adrenergic receptor（β3-AR） gene and diabetic foot in Chinese subjects.Methods The mutational genotype of Trp64Arg in β3-AR gene was measured by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in 110 patients with type 2 diabetes mellitus （T2DM group） [（58 with diabetic foot ulcer （DFU group） and 52 without （N-DFU group）] and 42 normal healthy subjects （normal control group）.The genotype （TT,TA or AA） and allele frequencies（T or A） were compared among groups.The anthro-pometry and metabolic indices were also compared between groups.Results （1） No significant deviation from Hardy-Weinberg equilibrium was found in the β3-AR Trp64Arg genotype frequency.In T2DM group,body mass index and homeostasis model assessment for insulin resistance index （HOMA-IR） in Arg allele were significantly higher than those without mutations allele （P =0.007 2,P=0.038 6）.（2） In T2DM group,Arg64 carriers had lower level of high density lipoprotein-cholesterol than those without this mutation （P=0.047 5）.（3）The frequency of Arg64 allele in DFU group was greatly higher than that of N-DFU group（x2=5.147,P〈0.05）,while no difference in β3-AR gene genotype and allele frequency was observed between T2DM group and normal control group（x2=0.067,P 〉0.05）.Conclusion β3-AR gene polymorphism is associated with diabetic foot.The Arg64 allele may be one of the risk factors of diabetic foot.

关 键 词：Β3肾上腺素能受体 基因多态性 糖尿病足 胰岛素抵抗 

分 类 号：R587.2[医药卫生—内分泌]