南极适冷菌Pseudomonas sp.P4-11抑菌活性物质的分离纯化与结构鉴定  

Separation, purification and structure identification of the antimicrobial substances from Antarctic psychrotrophic bacterium Pseudomonas sp. P4-11

作　　者：王红梅[1,2] 彭玉娇[1,2] 林学政[1] 

机构地区：[1]国家海洋局第一海洋研究所/国家海洋局海洋生物活性物质重点实验室,山东青岛266061 [2]齐鲁工业大学食品与生物工程学院,山东济南250353

出　　处：《广东农业科学》2014年第3期149-152,158,共5页Guangdong Agricultural Sciences

基　　金：海洋公益性行业科研专项(201005032-2)

摘　　要：以尖孢镰刀菌为指示菌,对南极适冷菌Pseudomonas sp.P4-11发酵液中的抑菌活性物质稳定性进行了研究.结果表明,在温度30～60℃和pH值6～10的范围内发酵液上清的抑菌活性稳定.通过抑菌活性追踪检测,利用乙酸乙酯萃取、硅胶柱层析及HPLC等方法对抑菌活性物质进行了分离纯化,获得了2个具有明显抑菌效果的化合物（T2-20-7-11-5,T2-20-7-11-7）,结构鉴定表明,二者均为壬基酚聚氧乙烯醚类化合物.利用扫描电镜对化合物T2-20-7-11-5抑制尖孢镰刀菌孢子的初步研究表明,该化合物可能主要作用于细胞壁或细胞膜,造成细胞内容物外泄,最终对孢子萌发以及菌丝体生长起到抑制作用.The antimicrobial substances of Antarctic psychrotrophic bacterium Pseudomonas sp. P4-l 1 were studied in this paper. The results of study on temperature and pH stability of bioactive substances of fermented culture showed that it had better stability at the range of 30-60℃ and pH 6-10. By bioassay-guided, two antimicrobial substances （T2-20-7-11-5 and T2-20-7-11-7） were isolated by ethyl acetate extraction, silica gel column chromatograph and HPLC. The structure of T2-20-7-11-5 and T2-20-7-11-7 were both identified as nonylphenol ethoxylates. The effect of T2-20-7-11-5 on the ultrastructure of Fusarium oxysporum spore was observed by scanning electron microscope （SEM）. The compound might have a major role in the cell wall or cell membrane, causing leakage of cell contents and consequently resulting in spore＇s death.

关 键 词：南极细菌 抑菌活性物质 分离纯化 结构鉴定 壬基酚聚氧乙烯醚 

分 类 号：Q936[生物学—微生物学]