Determination of tetraethyl ammonium by ion-pair chromatography with indirect ultraviolet detection using 4-aminophenol hydrochloride as background ultraviolet absorbing reagent  

作　　者：Chun-Miao Zou Hong Yu Miao-Yu Wang 

机构地区：[1]College of Chemistry and Chemical Engineering,Harbin Normal University and Key Laboratory of Photonic and Electronic Bandgap Materials,Ministry of Education

出　　处：《Chinese Chemical Letters》2014年第2期201-204,共4页中国化学快报（英文版）

基　　金：supported by the Natural Science Foundation of Heilongjiang Province(Grant No.B201307);the Ministry of Education of Heilongjiang Province(No.12531192);the Program for Scientific and Technological Innovation Team Construction in Universities of Heilongjiang Province(No. 2011TD010)

摘　　要：A method was developed for the determination of tetraethyl ammonium （TEA） by reversed-phase ion- pair chromatography with indirect ultraviolet detection, Chromatographic separation was achieved on a reversed-phase C18 column using background ultraviolet absorbing reagent - ion-pair reagent - organic solvent as mobile phase. The effects of the background ultraviolet absorbing reagents, detection wavelength, ion-pair reagents, organic solvents and column temperature on the determination method were investigated and the retention rules discussed. Results found that TEA could be successfully analyzed by using 0.7 mmol/L 4-aminophenol hydrochloride and 0.15 mmol/L 1-heptanesulfonic acid sodium mixed with 20% （v/v） methanol as mobile phase at a UV detection wavelength of 230 nm. Under these conditions, the retention time of tetraethyl ammonium was 2.85 min. The detection limit （S/N = 3） for TEA was 0.06 mg/L. The relative standard deviations （n = 5） for peak area and retention time were 0.35% and 0.02%, respectively. The method has been successfully applied to the determination of synthesized tetraethyl ammonium bromide. Recovery of tetraethyl ammonium after spiking was 99.1%.A method was developed for the determination of tetraethyl ammonium （TEA） by reversed-phase ion- pair chromatography with indirect ultraviolet detection, Chromatographic separation was achieved on a reversed-phase C18 column using background ultraviolet absorbing reagent - ion-pair reagent - organic solvent as mobile phase. The effects of the background ultraviolet absorbing reagents, detection wavelength, ion-pair reagents, organic solvents and column temperature on the determination method were investigated and the retention rules discussed. Results found that TEA could be successfully analyzed by using 0.7 mmol/L 4-aminophenol hydrochloride and 0.15 mmol/L 1-heptanesulfonic acid sodium mixed with 20% （v/v） methanol as mobile phase at a UV detection wavelength of 230 nm. Under these conditions, the retention time of tetraethyl ammonium was 2.85 min. The detection limit （S/N = 3） for TEA was 0.06 mg/L. The relative standard deviations （n = 5） for peak area and retention time were 0.35% and 0.02%, respectively. The method has been successfully applied to the determination of synthesized tetraethyl ammonium bromide. Recovery of tetraethyl ammonium after spiking was 99.1%.

关 键 词：Ion-pair chromatography Indirect ultraviolet detection Tetraethyl ammonium 4-Aminophenol hydrochloride 

分 类 号：O657[理学—分析化学]