纳米金光度法测定维生素B_1  

Determination of VB_1 by a Colorimetric Method Based on the Aggregation of Gold Nanoparticles

作　　者：王瑞勇[1] 范淑敏[1] 康小慧[1] 王瑞[1] 葛宝玉[1] 贾雪雷[1] 

机构地区：[1]郑州大学化学与分子工程学院,河南郑州450001

出　　处：《郑州大学学报（理学版）》2014年第1期83-87,共5页Journal of Zhengzhou University:Natural Science Edition

基　　金：国家自然科学基金资助项目;编号20905065

摘　　要：采用柠檬酸钠还原氯金酸的方法制得粒径约为13 nm的纳米金.在酸性环境中纳米金与维生素B1发生相互作用,纳米金产生凝聚,使其最大吸收峰发生红移,由此建立维生素B1的比色测定方法.优化了溶液pH、纳米金的浓度以及反应时间.在最佳条件下,方法的线性范围为5～ 60 ng/mL,检测限为0.74 ng/mL.用此方法成功分析了维生素B1片剂和注射液中维生素B1的含量.Gold nanopartieles were prepared by sodium citrate reduction method, and the diameter was a- bout 13 nm. In weakly acidic buffer medium, vitamin B1 （VB1） interacted with gold nanoparticles to form a binding product, resulting in a red shift of UV-vis absorption spectra, so that the concentration of VB1 could be determined. The experimental parameters were optimized with regard to pH, incubation time and the concentration of the gold nanoparticles. Under optimal experimental conditions, the linear range of the colorimetric sensor for VB1 was 5 -60 ng/mL, the corresponding limit of detection （3σ） was 0.74 ng/mL. The method was used for the determination of VB1 in tablet and injection, and the results were consistent with the method given in pharmacopoeia.

关 键 词：纳米金 维生素B1 光度法 

分 类 号：X831[环境科学与工程—环境工程]