慢性心房颤动心房肌胶原基因表达的芯片研究  

Collagen gene expression changes in atrial myocardium of chronic atrial fibrillation patients:microarray analysis of mRNA expression

作　　者：种朋贵[1] 曾祥君[2] 高鸿[1] 秦成名[3] 

机构地区：[1]贵阳医学院附属医院麻醉科,贵阳550001 [2]贵阳中医学院第二附属医院心胸外科 [3]湖北医药学院附属太和医院麻醉科

出　　处：《临床心血管病杂志》2014年第3期192-194,共3页Journal of Clinical Cardiology

摘　　要：目的:应用表达谱基因芯片技术研究慢性心房颤动(房颤)心房肌胶原基因表达的模式转变。方法:选择风湿性心脏病二尖瓣狭窄患者为研究对象,采集右心耳标本行表达谱芯片检测,获取胶原基因簇表达数据行主成份、显著差异基因、层级聚类和GO分类分析。结果:显著差异基因分析显示,人慢性房颤心房肌胶原簇基因差异表达的基因达14条,其中11条上调,3条下调;主成份分析和层级聚类表明,慢性房颤与窦性心律患者心房肌胶原基因表达模式明显不同;基因本体分析显示,胶原的合成、降解及胶原纤维的构成等生物学过程参与房颤形成。结论:房颤心房肌间质胶原纤维沉积增多,各型胶原比例失调,在房颤形成机制中具有重要意义。Objective：To assess atrial fibrillation （AF）-related collagen changes with cDNA microarray. Method：RNA samples extracted from right atrial specimens of 48 open heart surgery patients with rheumatic mitral stenosis, 24 with and 24 without AF, were sent for pooling microarray comparison. A list of collagen genes was curated via Gene-Ontology （GO） databases for microarray expression analysis. Result：We got a total of 14 collagen genes significantly differentially expressed, including 11 up-regulated genes and 3 down-regulated genes. All indi- vidual AF atria samples were clearly separated from the of SR atria samples respectively in the PCA and hierarchi- cal clustering. GO distribution based on biological process suggested that the majority of differential genes were involved in collagen fibril organization and collagen biosynthetic process and collagen metabolic process. Conclusion： Increased collagen fibers deposition and imbalance in various collagen component contributes to the development of AF.

关 键 词：心房颤动 胶原 基因芯片 主成份分析 层级聚类 

分 类 号：R541.7[医药卫生—心血管疾病]