HCN2与HCN4在风湿性心脏病二尖瓣狭窄患者并发心房颤动中的协同表达  

Coordinated expression of HCN2 and HCN4 in atrial fibrillation patients with mitral stenosis of rheumatic heart disease

作　　者：刘文洲[1] 周华富[1] 孙宇[1] 王俊龙[1] 赵春鹤[1] 冯旭[1] 杨涛[1] 钱俊[1] 

机构地区：[1]广西医科大学第一附属医院心胸外科,南宁530021

出　　处：《临床心血管病杂志》2014年第3期198-200,共3页Journal of Clinical Cardiology

基　　金：广西自然基金资助项目(No:2012GXNSFAA053094);广西科学攻关项目(No:桂科攻0816004-24)

摘　　要：目的:分析超极化激活环核苷酸调控通道基因家族-2(HCN2)基因及超极化激活环核苷酸调控通道基因家族-4(HCN4)基因在风湿性心脏病二尖瓣狭窄(风心二狭)患者并发心房颤动中的协同表达作用,探讨其并发心房颤动的机制。方法:收集43例风心二狭患者右心耳组织,实验组为并发心房颤动患者(AF组),对照组为窦性心律患者(SR组)。采用逆转录聚合酶链反应(RT-PCR)及荧光定量PCR(FQ-PCR)检测HCN2及HCN4的表达,分析两者的相关性。结果:RT-PCR及FQ-PCR结果均显示,AF组HCN2及HCN4表达量均高于SR组(P<0.01),AF组HCN2与HCN4表达上调率均呈现正相关(r1=0.697,P1<0.01;r2=0.659,P2<0.01)。结论:HCN2与HCN4的协同表达作用在风湿性心脏病二尖瓣狭窄患者并发心房颤动的发生发展中起重要的作用。Objective：To analyze the expressions and correlations of HCN2 and HCN4 in atrial fibrillation patients with mitral stenosis of rheumatic heart disease （RHD）, and to explore the possible adjustment mechanism of atrial fibrillation in RHD. Method： A total of 43 patients with RHD was divided into two groups： the atrial fibrillation group （AF） and the sinus rhythm group （SR）. The expressions of HCN2 and HCN4 were detected with reverse transeription-PCR （RT-PCR） and real-time fluores-cence quantitative PCR （FQ-PCR） ; then analyze the cor- relation between HCN2 and HCN4. Result：The levels of expressions of HCN2 and HCN4 in atrial fibrillation patients with mitral stenosis of RHD were not only higher than the SR group （P〈0.01）, but also have a positive correlation expressions between HCN2 and HCN4 （r1= 0. 697, P1 〈 0.01 ; r2 = 0. 659, P2 〈0.01）. Conclusion： The joint up-regulation function expressions of HCN2 and HCN4 play an important role in atrial fibrillation in RHD.

关 键 词：风湿性心脏病 二尖瓣狭窄 心房颤动 超级化激活环核苷酸调控通道基因家族-2 超级化激活环核苷酸调控通道基因家族-4 协同作用 

分 类 号：R541.2[医药卫生—心血管疾病]