外周血Th22和Th17细胞在急性心肌梗死患者中变化及临床意义  

Clinical significance of peripheral blood Th22,Th17 cells in diagnosis of acute myocardial infarction

作　　者：付才华 杜乃立[1] 张磊 王晓琦[1] 关玉庆[1] 王立启[1] 杜洋[3] 

机构地区：[1]山东大学附属济南市中心医院心内科,济南250013 [2]山东大学附属千佛山医院骨外科 [3]泰山医学院

出　　处：《临床心血管病杂志》2014年第3期236-240,共5页Journal of Clinical Cardiology

摘　　要：目的:探讨急性心肌梗死(AMI)患者外周血Th22和Th17细胞,及其细胞因子白细胞介素(IL)-22和IL-17的变化及临床意义。方法:冠心病患者(CHD)34例,其中AMI患者19例(AMI组)、稳定型心绞痛(SAP)患者15例(SAP组),另取15例冠状动脉造影正常者作为健康对照组(HC组);采用流式细胞技术检测各组外周血Th22和Th17细胞在CD4+T细胞中的比例;ELISA法检测血浆IL-22和IL-17浓度。结果:AMI组外周血Th22细胞(2.43±1.77)%、Th17细胞(2.80±1.15)%,均显著高于SAP组(0.92±0.26)%,(1.59±0.49)%及HC组(0.84±0.19)%、(1.35±0.32)%(均P<0.05),SAP组与HC组差异无统计学意义。AMI组血浆IL-22(33.74±7.25)pg/ml、IL-17(24.95±8.86)pg/ml浓度均显著高于SAP组(26.95±3.19)pg/ml,(18.86±5.65)pg/ml及HC组(24.17±2.55)pg/ml、(14.63±2.57)pg/ml(均P<0.05),SAP与HC组差异无统计学意义。AMI组患者的Th22细胞比例与Th17细胞比例(r=0.525,P<0.05)、IL-22浓度(r=0.771,P<0.01)均呈显著正相关,AMI组的Th17细胞比例与IL-17浓度也呈显著正相关(r=0.627,P<0.01)。结论:外周血Th22、Th17细胞及其相关细胞因子在AMI的发生中起重要作用。Objective：To investigate the change and clinical significance of peripheral blood Th22, Th17 ceils as well as cytokines interleukin-22 （IL-22） and interleukin-17 （IL-17） in acute myocardial infarction （AMI）. Method..A total of 34 coronary heart disease patients conformed by coronary angiography was divided into AMI group （n=19） and stable angina pectoris （SAP） group （n= 15）. Another 15 subjects with normal angiography results were selected as healthy control （HC） group. The frequency of Th22 and Th17 in CD4＋T cells were detected by flow cytometry and the concentration of plasma IL-22 and IL-17 were measured by ELISA. Result： Th22, Th17 cells were significantly increased in AMI group [（2.43 ± 1.77）%, （2.80± 1.15）%] compared with SAP group [（0.92±0.26）%, （1.59±0.49）%] and HCgroup [（0.84±0.19）%, （1.35±0.32）%] （all P〈0. 05）. There was no significant difference between SAP and HC group. The concentration of plasma IL-22 and IL-17 was also significantly elevated in AMI group [（33.74± 7.25）pg/ml, （24.95± 8.86）pg/ml] compared with SAP group [（26.95±3.19）pg/ml, （18.86±5.65）pg/ml] and HC group [（24.17±2.55）pg/ml, （14.63±2.57）pg/ml] （all P〈0. 05）. There was also no difference between SAP and HC group. The frequency of Th22 was positively correlated with Thl7 （r=0. 525, P〈0.05） as well as IL-22 （r=0. 771, P〈0.01） in AMI group. The frequency of Thl7 was also positively correlated with IL-17 （r= 0. 627, P〈0.01） in AMI group. Conclusion：Th22, Thl7 cells and cytokines in peripheral blood play an important role in the occurrence of AMI.

关 键 词：急性心肌梗死 TH22细胞 TH17细胞 白细胞介素-22 白细胞介素-17 

分 类 号：R542.2[医药卫生—心血管疾病]