血卟啉和核黄素荧光寿命的测量  

Fluorescence lifetime measurement of haematoporphyrinand lactoflavin

作　　者：立群[1] 王文耀[1] 高福源[1] 施阿英[1] 邱佩华[1] 江寿平[2] 连少辉[2] 

机构地区：[1]中国科学院上海光学精密机械研究所,上海201800 [2]中国科学院上海生物化学研究所,上海200031

出　　处：《光学学报》1991年第4期381-384,共4页Acta Optica Sinica

摘　　要：本文选用了波长为1.054μm的磷酸盐钕玻璃锁模激光器输出的单个PS激光脉冲,经KDP晶体倍频后的绿光(λ=0.527μm)做激发光源。用条纹相机测定了血卟琳、核黄素有机生物大分子的激发单态S_1的寿命。并就氧分子的猝灭效应对寿命的影响进行了初步讨论。In our experiment described in this paper, the single pulse of the picosecond laser (λ=0.527μ) from bhe output of a frequency-doubled mode-locked phosphate Nd glass laser at λ=1.054μm was used as the exciting light source. The lifetimes of the excited singlet states S1 of the organic biological macromolecules haema-toporphyrin and lactoflavin were measured with streak camera. The quenching effect of oxygen molecules on the lifetimes was discussed preliminarily.

关 键 词：血卟啉 核黄素 荧光寿命 条纹相机 

分 类 号：Q563.2[生物学—生物化学]