脊尾白虾的性腺发育及组织结构观察  

Histological study on the gonadal development of Exopalaemon carinicauda (Holthuis,1950)

作　　者：栗治国[1,2] 张成松[1] 李富花[1] 相建海[1] 

机构地区：[1]中国科学院实验海洋生物学重点实验室,山东青岛266071 [2]中国科学院大学,北京100049

出　　处：《水产学报》2014年第3期362-370,共9页Journal of Fisheries of China

基　　金：国家科技支撑计划(2011BAD13B01);国家自然科学基金项目(41376165);虾产业体系专项(CARS-47)

摘　　要：为系统研究脊尾白虾的性腺发育及组织学特征,采用常规的石蜡切片及H.E染色方法对脊尾白虾的性腺发育及其组织结构进行观察。结果表明,脊尾白虾的雌性生殖系统由卵巢、输卵管及排卵孔组成。卵子发生经历了卵原细胞、卵黄合成前期卵母细胞、内源性卵黄合成期卵母细胞、外源性卵黄合成期卵母细胞,最后发育为成熟的卵母细胞。卵巢发育可分为增殖期、小生长期、大生长期、成熟期及产后恢复期。脊尾白虾雄性生殖系统由精巢、输精管及排精孔组成。精巢由生精小管构成,不同生精小管内精子发育可不同步。精子发生经历了精原细胞、初级精母细胞、次级精母细胞、精细胞,最后发育为精子。输精管可分为前、中、后输精管及末端壶腹,精荚在输精管中形成。Ridgetail white prawn（ Exopalaemon carinicauda）is an important commercially exploited species in China, however, its speedy cultural development suffered the seedling shortage because of the absence of standard protocol in artificial seedling. To provide the basic knowledge needed in artificial reproduction, a histological study on the gonadal development of E. carinicauda was performed by the paraffin section technique and HE staining method. The results show that the female reproductive system consists of ovary, oviduct and oviporus. Oogenesis could be classified into five different phases and the ovary development could be divided into five stages accordingly. The average gonad index varied from 0 to 10%. The female could spawn several times in the reproductive season. The male reproductive system is composed of testis, vas deferens and male gonopore. The testis consists of many seminiferous tubules, in which there are numerous sperms at different development stages. The spermatogenesis could be classified into five phases, i. e., spermatogonium,primary spermatocyte, secondary spermatocyte, spermatid and sperm. The vas deferens where the spermatophore forms could be separated into anterior, medial,posterior and terminal segments.

关 键 词：脊尾白虾 性腺发育 组织结构 

分 类 号：Q136[生物学—普通生物学] S917.4[农业科学—水产科学]