重症急性胰腺炎大鼠血清高迁移率族蛋白-1对胰腺腺泡细胞死亡方式的影响  

Effect of High Mobility Group Box-1 on The Death Pathways of Pancreatic Acinar Cell in Rat with Severe Acute Pancreatitis

作　　者：王俊[1] 蔡治方[1] 刘尧[1] 兑丹华[1] 

机构地区：[1]遵义医学院附属医院普通外科,贵州遵义563003

出　　处：《中国普外基础与临床杂志》2014年第3期295-299,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：贵州省科学技术基金项目〔项目编号:黔科合J字(2008)2200号〕~~

摘　　要：目的探讨重症急性胰腺炎(SAP)大鼠血清高迁移率族蛋白-1(HMGB1)水平对胰腺腺泡细胞胀亡的影响。方法将32只SD大鼠随机分为假手术组(SO组,n=8)和SAP组(n=24)。SO组大鼠开腹后仅翻动肠管,SAP组大鼠采用胆胰管内逆行注射3%牛磺胆酸钠的方法建立SAP模型。建模成功后,将SAP组大鼠随机均分为6、12及24 h组,每组8只,分别于上述时点处死大鼠,取静脉血及胰腺组织,行HE染色以观察胰腺组织的病理学改变,行ELISA法检测血清中的HMGB1浓度,以流式细胞仪检测胰腺腺泡细胞的胀亡百分比。结果 SO组大鼠的胰腺腺泡结构完整,偶见单个炎症细胞浸润。SAP-6 h组可见胰腺腺泡肿胀、间质水肿,炎症细胞浸润;SAP-12 h组及SAP-24 h组见胰腺病理学损伤加重,部分腺泡细胞坏死,间质血管瘀血,局灶坏死,且随时间延长病理学损伤加重。3个SAP亚组大鼠的血清HMGB1浓度和胰腺腺泡细胞胀亡百分比均高于SO组(P<0.01),且随时间延长,SAP大鼠的血清HMGB1浓度和胰腺腺泡细胞胀亡百分比均增高(P<0.05)。24 h内SAP大鼠的血清HMGB1浓度与胰腺腺泡细胞胀亡百分比呈正相关(r=0.846,P<0.01)。结论 SAP时,HMGB1在介导炎症反应的同时,可能诱导胰腺腺泡细胞胀亡,从而参与SAP的病理生理过程。Objective To explore the effect of the serum high mobility group box-1 （HMGB 1） on oncosis of pancreatic acinar cells in the rat with severe acute pancreatitis （SAP）. Methods Thirty-two healthy SD rats were rand-omly divided into 2 groups ： sham operation group （SO group, n=8） and SAP group （n=24）. Rats of SO group were only flipped the intestinal canal after laparotomy, but rats of SAP group were induced by retrograde injection of 3% sodium taurocholate into bilio-pancreatic duct in addition. Rats of SO group were sacrificed at 6 hours after operation, and rats of SAP group were sacrificed at 6 （SAP-6 hour group, n=8）, 12 （SAP-12 hour group, n=8）, and 24 hours （SAP-24 hour group, n=8） after operation respectively. Pancreatic tissues were stained by HE to observe pathological changes. Serum HMGB1 was measured by ELISA, and the oncosis percentage of pancreatic acinar cells was examined by flowcytometry. Results Pathological results showed that structural integrity was observed in pancreatic aeinar, and occasionally a single inflammatory cell infiltration was observed in rats of SO group. Swelling, interstitial edema, and inflammatory cell infiltration were observed in rats of SAP-6 hour group. Some necrosis of pancreatic acinar cell, stromal vascular cong- estion, and focal necrosis were observed in rats of SAP-12 hour group and SAP-24 hour group, which the pathological damage were worse over time. Levels of serum HMGB1 and oncosis percentages of pancreatic acinar cells in rats of3 SAP subgroups were all higher than those of SO group （P 〈 0. 01）, and the 2 kinds of indexes both increased over time （P〈 0. 05）. There was positive correlation between concentration of serum HMGB 1 and oncosis percentages of pancreatic acinar cells in SAP rat during 24 hours after operation （r =0. 846, P 〈 0.01）. Conclusion HMGB 1 seems to play an impor-tant role in SAP by inducing oncosis of pancreatic acinar cells when inducing inflammatory reaction in rat with SAP.

关 键 词：重症急性胰腺炎 高迁徙率族蛋白-1 细胞胀亡 流式细胞术 

分 类 号：R657.51[医药卫生—外科学]