Apelin在大鼠中枢痛觉调制中的作用及其机制的实验思路分析  

Experimental analysis on the effects and mechanism of Apelin in rats' central pain modulation

作　　者：杨宇[1] 邢爱平[2] 姜楠[1] 冉梦璇 肖红[1] 姜晓龙[1] 

机构地区：[1]沈阳医学院基础医学院,辽宁沈阳110034 [2]沈阳医学院公共卫生学院,辽宁沈阳110034

出　　处：《中国医药科学》2014年第4期26-28,共3页China Medicine And Pharmacy

摘　　要：目的对大鼠中枢痛觉Apelin的调制作用及其机制进行实验研究,从而探讨Apelin在机体中枢痛敏作用,为降低机体痛觉提供可靠依据,提高临床疗效及生活质量。方法利用注射器通过自制套管对大鼠侧脑室进行相应物质注射,即实验组注射Apelin、对照组A注射生理盐水、对照组B注射盐酸吗啡,之后10、20、30、40、50、60min分别测量TFL%,给予统计学分析后得出结论。结果实验组大鼠痛阈较注射前显著降低,且于注射后30min达到最低值;对照组A大鼠较实验前痛阈无显著变化;对照组B大鼠较实验前痛阈显著上升,且注射后30min升至最高,三组大鼠痛阈变化情况对比结果具有统计学意义(P<0.05)。结论 Apelin可显著升高机体痛敏反应,因此有助于研究临床某些镇痛药物无法有效镇痛的原因以提高镇痛效果。Objective To investigate the modulation effects and mechanism of apelin in rats＇ central pain via experiment,so as to discuss the effect of apelin in central hyperalgesia and provide reliable evidence for lowering pain and thus improve clinical curative effects and life quality. Methods Syringes were used to inject relevant solutions to rats＇ lateral ventricle via self-made cannula.Experimental group was injected with apelin; control group A was injected with normal saline; control group B was injected with morphine hydrochloride.TFL% in the three groups were measured in 10min, 20min, 3Omin, 40min, 50min and 60min respectively.The conclusion was drawn based on statistical analysis. Results The pain threshold for rats in experimental group was significantly lower than that before the injection,and it reached the minimum level 30min after the injection; there was no significant change of pain threshold for rats in control group A after the injection; the pain threshold for rats in control group was higher than that before the injection, and it reached the maximum level 30min after the injection. The differences of changes of pain threshold in the three groups were statistically signifieant（P 〈 0.05）. Conclusion Apelin has the effect of significantly improving hyperalgesia, which therefore helps clinically study reasons for some analgesic drugs which fail to show effects and thus improve analgesic effects.

关 键 词：APELIN 中枢痛觉调制 甩尾测痛法 

分 类 号：R402[医药卫生—临床医学]