慢性氟中毒对大鼠仔代神经系统发育的影响及维生素E的拮抗作用  

Effects of chronic fluorosis on neurobehavioral development in offspring of rats and antagonistic effect of Vitamin E

作　　者：魏娜[1] 董阳婷 王娅[1] 官志忠 

机构地区：[1]贵阳医学院病理学教研室,550004 [2]贵州省分子生物学重点实验室

出　　处：《中华地方病学杂志》2014年第2期125-128,共4页Chinese Journal of Endemiology

基　　金：国家自然科学基金（81160335）;科技部项目（2013BA105803、2010DFB30530）;贵州省科技厅国际合作项目（黔科合外G字[2011]7014号）;贵州省科技计划（黔科合计Z字[2012]4010）

摘　　要：目的观察慢性氟中毒对大鼠仔代神经行为发育和学习记忆能力的影响及维生素E（Vi（E）的拮抗作用。方法选择1月龄SD大鼠45只，其中雌鼠30只，雄鼠15只。按体质量采用随机数字表法分为对照组、染氟组及VitE拮抗组，每组15只（雌鼠10只，雄鼠5只）。通过饮用水加氟化钠和VitE灌胃（各组的含氟量分别为〈1．50、50mg／L，含VitE量分别为0、0、50mg／kg）处理5个月，然后按雌雄2：1合笼交配。观察母鼠生育指标（受孕率、妊娠时间、产仔数、每窝活胎数）；自然分娩后仔代神经行为发育指标（平面翻正、空中翻正、断崖回避和听觉惊愕时间）。采用Morris水迷宫实验观察出生30d仔鼠学习记忆能力（平均逃避潜伏期、第5天穿越平台次数）。结果染氟组和VitE拮抗组与对照组比较．其受孕率、妊娠时间、产仔数、每窝活胎数未见明显改变（P均〉0．05）；染氟组和VitE拮抗组仔鼠完成平面翻正、空中翻正的时间[（8．1±1．4）、（7．9±1．5）d，（17．7±2．3）、（17．2±1．8）d]与对照组[（6．4±1．8）、（15．1±1．7）d]比较显著延迟（P均〈0．05），完成断崖回避和听觉惊愕的时间未见明显改变（P均〉0．05）；与对照组、VitE拮抗组[（31．74±17．78）、（34．97士15．44）s，（4．50±2．51）、（3．80±1．87）次]比较，染氟组[（42．03±16．45）s，（2．20±1．62）次]仔鼠水迷宫平均逃避潜伏期延长，第5天穿越平台次数减少（P均〈0．05）。结论慢性氟中毒对仔代神经行为发育和学习记忆有一定损害作用．经一定剂量VitE处理，可改善慢性氟中毒的损伤作用。Objective To study the effect of chronic fluorosis on neurobehavioral development, the ability of learning and memory in offspring of rats, and the antagonistic effect of antioxidant Vitamin E （Vit E）. Methods According to body weight, forty-five 1-month-old Sprague-Dawley（SD） rats of 30 females and 15 males were divided into three groups by random number table, including control group, fluorosis and Vit E antagonistic groups （15 rats with 10 females and 5 males in every group ）. Five months after establishing the animal model with chronic fluorosis and Vit E gavage treatments （fluoride 〈 1, 50, 50 mg/L, respectively; Vit E 0, 0, 50 mg/kg, respectively）, the rats were mated in 2 ： 1 proportion of female ： male in different groups, respectively. The fertility index of female and neurobehavioral development indicators in offspring were observed. Spatial learning and memory of offspring after birth for 30 d were evaluated by using Morris Water Maze test. Results The female fertility index exposed to fluorosis and Vit E were not significantly different as compared to those of control group（all P 〉 0.05）; in contrast to control groups[ （6.4± 1.8）, （15.1± 1.7 ）d], the time that completed the surface righting reflex [（8.1± 1.4）, （7.9 ± 1.5）d] and the air righting reflex [（17.7±2.3）, （17.2± 1.8）d] were delayed in the offspring in fluorosis and Vit E antagonistic groups（all P 〈 0.05） ; the completed avoidance precipice reflex and the auditory consternation did not changed significantly（all P 〉 0.05）; In addition, compared with control and Vit E antagonistic groups [ （31.74 ± 17.78）, （34.97 ± 15.44）s, （4.50 ± 2.51）, （3.80± 1.87）time], the average escape latency and exploration platform at five days were decreased in 30 d offspring of fluorosis group[ （42.03 ± 16.45）s, （2.20 ± 1.62）time]. Conclusion Neurobehavioral development as well as learning and memory ability in rat offspring are impaired by long-term exposure to fluori

关 键 词：氟化物中毒 神经行为发育 学习 记忆 

分 类 号：R599.1[医药卫生—内科学]