Difference in apoptosis-associated genes expression profiling and immunohistology analysis between Kashin-Beck disease and primary osteoarthritis  

作　　者：Shixun Wu Chen Duan Feng Zhang Robert Pierce McKenzie Jingjing Zheng Umer Farooq Yidong Bai Xiong Guo 

机构地区：[1]School of Public Health, College of Medicine, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi’an Jiaotong University [2]Department of Orthopedics Surgery, The First Affiliated Hospital, College of Medicine, Xi’an Jiaotong University [3]Department of Cellular and Structural Biology, University of Texas Health Sciences Center at San Antonio [4]Department of Community Medicine, Ayub Medical College

出　　处：《Chinese Science Bulletin》2014年第9期833-839,共7页

基　　金：supported by the National Natural Science Foundation of China(30972556);the‘‘13115’’major program of the Technology Science Innovation Project of Shaanxi Province(2009ZDKG-79)

摘　　要：Kashin-Beck disease(KBD)is a chronic and deformed endemic osteoarthritis,without fully known etiology.As compared to primary osteoarthritis(POA),its pathogenesis still exists controversial.We performed this study to discriminate the difference in genes expression involved in apoptosis from KBD cartilages and POA cartilages.Microarray analysis and Ingenuity Pathway Analysis(IPA)were used to identify the molecular mechanisms/canonical pathways implicated in KBD arthritis.Immunohistochemistry staining was carried out to detect tissue distribution of PDCD5 and EGR1 in the knee cartilages from KBD and POA.The 23 up-regulated apoptosis-related genes with[2-fold change were identified.‘‘Role of Macrophages,Fibroblasts,and Endothelial Cells in Rheumatoid Arthritis’’signaling was screened as the most relevant pathway through IPA.The 8 differentially regulated genes were verified by qRT-PCR analysis.Programmed cell death 5 in middle and deeper zones and early growth response 1 in superficial and middle zones showed protein overexpressed in KBD cartilage samples comparing to those in POA cartilage samples.Data indicates a higher expression level of apoptosis-related functional genes in KBD articular cartilage compared with POA articular cartilage.Kashin-Beck disease （KBD） is a chronic and deformed endemic osteoarthritis, without fully known eti- ology. As compared to primary osteoarthritis （POA）, its pathogenesis still exists controversial. We performed this study to discriminate the difference in genes expression involved in apoptosis from KBD cartilages and POA car- tilages. Microarray analysis and Ingenuity Pathway Ana- lysis （IPA） were used to identify the molecular mechanisms/canonical pathways implicated in KBD arthritis. Immunohistochemistry staining was carried out to detect tissue distribution of PDCD5 and EGR1 in the knee cartilages from KBD and POA. The 23 up-regulatedapoptosis-related genes with 〉2-fold change were identi- fied. ＂Role of Macrophages, Fibroblasts, and Endothelial Cells in Rheumatoid Arthritis＂ signaling was screened as the most relevant pathway through IPA. The 8 differen- tially regulated genes were verified by qRT-PCR analysis. Programmed cell death 5 in middle and deeper zones and early growth response 1 in superficial and middle zones showed protein overexpressed in KBD cartilage samples comparing to those in POA cartilage samples. Data indi- cates a higher expression level of apoptosis-related func- tional genes in KBD articular cartilage compared with POA articular cartilage.

关 键 词：细胞凋亡相关基因 大骨节病 骨关节炎 原发性 免疫组化 表达谱 RT-PCR分析 程序性细胞死亡 

分 类 号：R684[医药卫生—骨科学]