杨树扩展蛋白基因家族的生物信息学分析  

Bioinformatics analysis of expansin gene family in poplar genome

作　　者：李昊阳[1] 施杨[1] 丁亚娜[1] 徐吉臣[1] 

机构地区：[1]北京林业大学林木育种国家工程实验室

出　　处：《北京林业大学学报》2014年第2期59-67,共9页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(30771516)

摘　　要：扩展蛋白是植物细胞壁的重要组成部分,广泛存在于植物基因组中,具有疏松细胞壁组分、增加细胞壁柔韧性的作用,在植物的生长发育及抗性等多个方面起着重要的作用。全基因组序列分析显示,杨树中的扩展蛋白基因家族包含36个成员,分属于EXPA、EXPB、EXLB和EXLA 4个亚家族。系统生物信息学分析表明,杨树扩展蛋白基因具有保守的序列特征和稳定的蛋白结构,包括9种不同的内含子起源模式以及6个高频密码子。基因表达分析显示,该家族基因在杨树木材形成相关的组织/器官中表达丰度较高,对林木生殖发育也起着重要的作用。研究结果展示了杨树扩展蛋白基因家族的基本信息和特点,为深入研究杨树扩展蛋白基因的功能搭建平台。As an important component of plant cell wall, expansins widely present in plant genome and have unique ＂loosening＂ effects and are thought to function in plant growth, development, plant resistance, etc. Whole genome sequencing of poplar reveals it containing 36 expansin genes classified in to 4 subfamilies （EXPA, EXPB, EXLA and EXLB）. Systematic bioinformatics analysis showed that poplar expansin genes had the conserved apparent sequence features and stable protein structure. It obviously displayed 9 different introns originate modes and 6 high-frequency codons. Transcriptome analysis showed that the expansin genes expressed highly in poplar wood forming tissues or organs, and were associated with the reproductive development. The results here displayed the basic information of poplar expansin superfamily which could be a good platform for the gene function study in future.

关 键 词：杨树 扩展蛋白 细胞壁 基因家族 生物信息学 

分 类 号：S792.11[农业科学—林木遗传育种] Q943.2[农业科学—林学]