他克莫司对肾移植受者血脂的影响及其与GNB3基因C825T多态性的相关性研究  

Effect of tacrolimus on blood lipid after renal transplantation and association of G protein beta 3 subunit （GNB3） gene C825T polymorphism to serum lipid levels

作　　者：吴存造[1] 杨亦荣[1] 潘晓东[1] 郭勇[1] 倪晓洁[1] 孙贤斌[1] 夏鹏[1] 

机构地区：[1]温州医科大学附属一院移植科,325003

出　　处：《中华器官移植杂志》2014年第3期129-132,共4页Chinese Journal of Organ Transplantation

基　　金：浙江省自然科学基金（LY12H10004）

摘　　要：目的研究他克莫司血药浓度对肾移植受者血脂的影响及其与G蛋白β3亚单位（GNB3）基因825T等位基因（GNB3C825T）多态性的相关性。方法81例肾移植受者依据他克莫司浓度分为普通浓度组（31例）和低浓度组（50例），免疫抑制方案均为联合应用甲泼尼龙（MP）、他克莫司、吗替麦考酚酯（MMF）及巴利昔单抗。统计受者移植后1、3、6和12个月的血清总胆固醇和甘油三脂水平；并且利用快速的序列特异性聚合酶链反应方法检测人GNB3C825T单核苷酸多态性，分析GNB3C825T多态性与他克莫司治疗后高血脂发生率的关系。结果两组术前总胆固醇和甘油三酯水平的差异无统计学意义（P〉0．05），排除发生急性排斥反应的受者后，两组术后3、6、12个月时甘油三脂水平的差异有统计学意义（P〈0．05），两组血胆固醇水平的差异无统计学意义（P〉0．05）。81例中，T／C基因型者高甘油三脂血症发生率为22．0％（9／41），C基因型者为27．3％（6／22），T基因型者为33．3％（6／18），高甘油三脂血症发生情况与GNB3基因多态性无明显相关（P=0．647）。结论他克莫司影响。肾移植术后的甘油三酯水平，与其浓度正相关；高甘油三脂血症发生率与GNB3C825T单核苷酸多态性相关不显著。Objective To study the effect of Tacrolimus on blood lipid after renal transplantation, and the relationship between C825T polymorphism in G protein beta 3 subunit （GNB3） gene and serum lipid levels. Method Eighty-one cases of recipients patients after renal transplantation were divided into two groups in terms of Tacrolimus concentration： normal blood concentration group （group A） and low blood concentration group （group B）. The serum lipid levels at 1st, 3rd, 6th, and 12th month after renal transplantation were measured. Genotype was determined by the simple sequence-specific primer polymerase chain reaction （SSP-PCR）. Result The percentage of patients with hypertriglyceride in group A was significantly higher than in group B during the one- year follow-up period. There was significant difference between the two groups in the serum triglyceride levels but no difference in the serum cholesterol levels. The 825C/T polymorphism in the GNB3 gene was not associated with hypertriglyceride in renal transplantations in Wenzhou. Conclusion The serum triglyceride levels in renal transplantations in Wenzhou was associated with the Tacrolimus concentration, and the incidence of hypertriglyceride is not associated with the 825C/T polymorphism in the GNt33 gene.

关 键 词：肾移植 他克莫司 高胆固醇血症 高甘油三酯血症 

分 类 号：R699.2[医药卫生—泌尿科学]