类风湿关节炎湿热痹阻型患者血清和滑膜液IL-17与ESR、CRP的相关性研究  

Correlation Study between Interleukin-17 and ESR and CRP in Serum and the Synovial Fluid of Rheumatoid Arthritis Patients of Accumulated Dampness-heat Obstruction in Joints Syndrome

作　　者：刘德芳[1] 晏姣[1] 郭明阳[1] 王超[2] 呼永河[1] 杨敏[1] 贠明东[1] 罗勇[1] 张俊[1] 李华[3] 

机构地区：[1]中国人民解放军成都军区总医院中西医结合风湿科,成都610083 [2]中国人民解放军成都军区总医院中心实验室,成都610083 [3]中国人民解放军成都军区总医院肿瘤科,成都610083

出　　处：《中国中西医结合杂志》2014年第3期272-275,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金面上项目(No.81072455);四川省卫生厅课题资助项目(No.120573)

摘　　要：目的通过检测类风湿关节炎(rheumatoid arthritis,RA)湿热痹阻型患者血清和滑膜液中白细胞介素-17(IL-17)表达水平,及炎性活动指标血沉(ESR)、C反应蛋白(CRP)的相关性分析,探讨IL-17在RA发病中的作用。方法选择2011年1月—2013年1月成都军区总医院中西医结合风湿科住院的RA湿热痹阻型患者90例(28例伴有膝关节积液)作为研究对象,以30名健康体检者作为健康对照组,以30例膝关节积液的骨性关节炎(OA)患者作为滑膜液对照组,采用ELISA法检测血清和滑膜液中IL-17表达水平,及RA患者ESR、CRP含量,将IL-17水平与ESR、CRP进行相关性分析。结果 RA湿热痹阻型患者血清中IL-17表达水平较健康对照组明显升高,差异均有统计学意义(P<0.05);RA滑膜液较RA血清和OA滑膜液中IL-17表达水平明显升高,差异有统计学意义(P<0.05);RA血清IL-17表达与炎性活动指标ESR、CRP无相关性(r=0.092,-0.082;P>0.05);RA滑膜液IL-17表达与炎性活动指标ESR、CRP亦无相关性(r=0.113,-0.034;P>0.05)。结论 IL-17作为Th17细胞的主要效应性细胞因子,RA湿热痹阻型患者不同病程中血清和滑膜液中IL-17的表达水平皆明显升高,但与ESR、CRP无相关性,说明IL-17参与了RA的发生发展,但具体作用机制还需更多大样本研究证实。Objective To probe the function of interleukin-17 （IL-17） in rheumatoid arthritis （RA） patients of accumulated dampness-heat obstruction in joints syndrome （ADOJ S） by detecting lev- els of IL-17 in serum and the synovial fluid and analyzing its correlation with erythrocyte sedimentation rate （ESR） and C reactive protein （CRP）. Methods From January 2011 to January 2013, recruited were 90 RA inpatients of ADOJS at Department of Integrative Medical Rheumatism, General Hospital of Chengdu Military Region, of which 28 patients had knee joint effusion. Besides, 30 healthy volunteers who received physical examination at our hospital were recruited as the normal control group, and 30 patients with osteoarthritis （OA） who had knee joint effusion were recruited as the synovial fluid control group. The expression levels of IL-17 in serum and the synovial fluid were detected by enzyme linked immu- nosorbent assay （ELISA）, and contents of ESR and CRP were detected in RA patients. Then correlation analyses were performed between levels of IL-17 and contents of ESR and CRP. Results Compared withthe normal serum control group, the expression levels of IL-17 in serum of RA patients significantly in- creased （P 〈0.05）. Compared with the serum of RA patients and the synovial fluid of OA patients, the expression levels of IL-17 in the synovial fluid of RA patients significantly increased （P 〈0.05）. The ex- pression levels of IL-17 in serum of RA patients were not correlated with ESR or CRP （r =0.092, -0.082 ; P 〉0.05）, and the expressional levels of IL-17 in the synovial fluid of RA patients were not correlated with ESR or CRP （r =0. 113, -0.034; P 〉0.05）. Conclusions IL-17 was the main effector cytokine of Th17 cells. The expressional levels of IL-17 significantly increased in serum and the synovial fluid of RA pa- tients of ADOJS, but with no correlation to ESR or CRP. It indicated that IL-17 participated in the occur- rence and development of RA. Concrete mechanisms needed to b

关 键 词：类风湿关节炎 湿热痹阻证 白细胞介素17 红细胞沉降率 C-反应蛋白 

分 类 号：R593.22[医药卫生—内科学]