双侧后肢压迫大鼠严重挤压伤动物模型  

The experimentaI model of severe crush injury in rats by two hindlimbs compression

作　　者：宋小兵[1] 赵剡[1] 沈俊[1] 王翔[1] 潘正启[1] 杨奇盛[1] 

机构地区：[1]武汉大学中南医院急救中心,武汉430071

出　　处：《中华急诊医学杂志》2014年第3期289-293,共5页Chinese Journal of Emergency Medicine

基　　金：湖北省自然科学基金（2011CDB506）

摘　　要：目的探索可重复的严重大鼠挤压综合征模型。方法武汉大学中南医院动物实验中心，清洁级雄性SD大鼠50只，随机（随机数字法）分为5组，麻醉后用3．5kg的重物按双侧后肢特殊槽式（sP组）、双侧后肢常规压迫（NM组）及单纯对照不压迫（SHAM组）6h，再灌注3h（每组10只大鼠）。所有大鼠在压迫开始前和再灌注3h后测量动脉压、血乳酸、K＋，CK，BUN，CRN及MB，相应的局部肌肉及肾脏进行病理切片检查。另外，进行相同的试验方法，监测观察双侧后肢常规压迫（D组）、双侧后肢特殊槽式（E组）生存率72h。采用SPSS17．0统计软件进行统计分析，重复测量数据的方差分析分析多组之间差异，Kaplan-Meier方法分析生存率。结果双侧后肢槽式组压迫较常规压迫组和单纯对照组增高了血乳酸（F=39．626，P〈0．05），AST（F=24．965，P〈0．05），ALT（F=19．096，P〈0．05），BUN（F=7．938，P〈0．05），CR（F=14．787，P〈0．05）及MB（F=16．840，P〈0．05）的水平，组织学标本中发现细胞损伤和缺血一再灌注损伤，双侧后肢卡槽式压迫在24h后导致潜在的急性肾小管坏死，常规式压迫组大鼠病死率20％，双侧后肢槽式挤压的病死率可达90％（P〈0．05）。结论该模型是一个有效的大鼠严重挤压综合征模型，有应用价值。Objective To investigate a reproducible model of severe crush injury （CI） in rats. Methods A total of 50 clean grade male SD rats were randomly （random number） divided into 5 groups. Both hindlimbs of anesthetized rats were compressed by blocks weighing 3.5 kg, for 6 hours and followed by 3 hours of reperfusion on a specially notched device （ group SP, n = 10）, ordinary compression （ group NM, n = 10） and simple control （ group SHAM, n = 10）. Arterial tension, serum lactate, and potassium （ K~ ） , serum myoglobin （MB） , aspartate transferase （AST） and alanine transferase （ ALT）, BUN and Cr were measured at 10 minutes after eannulaton, and 3 hours after release from compression. Muscles and kidneys were evaluated morphologically. Group D and E were treated in the same way and were observed for 72 h to get the survival rate of the NM group and the specially notched compression group. The SPSS 17. 0 statistical software was used for statistical analysis, repeated-measures ANOVA analysis for the differences between groups, Kaplan Meier-estimator for survival analysis. Results The Specially notched compression produced a greater increase in serum lactate （ F = 39. 626, P 〈 0. 05 ）, AST （ F = 24. 965, P 〈 0. 05 ）, ALT （ F = 19.096, P〈0.05）, BUN （ F=7.938, P〈0.05）, CR （F=14.787, P〈0.05） and MB （F= 16. 840, P 〈0.05） by the end of experiment than NM group and simple control group. The direct cellulardamage and ischemia-reperfusion injury were found under microscope. In crush injury caused by specially notched compression there was acute tubular necrosis found at 24 hours ＇after injury. Mortality rate in the NM group was 20%, whereas mortality rate reached 90% in rats with specially notched compression （P 〈 0. 05 ）. Conclusions It successfully developed a severe crush injury model in experimental rats, suggesting it is worthwhile to popularization.

关 键 词：挤压伤 动物模型 大鼠 压迫 再灌注 后肢 肌肉 肾脏 

分 类 号：R-332[医药卫生]