光动力疗法建立大鼠视网膜静脉阻塞模型  

Photodynamic Development of Experimental Retinal Vein Occlusion of Rats

作　　者：邹红[1] 黎蕾[2] 缪晚虹[1] 

机构地区：[1]上海中医药大学附属曙光医院眼科,上海市201203 [2]复旦大学附属眼耳鼻喉科医院眼科

出　　处：《中国激光医学杂志》2013年第6期303-307,354,355,共7页Chinese Journal of Laser Medicine & Surgery

基　　金：上海市卫生局青年科研基金资助课题(2010Y125)

摘　　要：目的建立SD大鼠视网膜静脉阻塞模型。方法成年雄性SD大鼠20只,双眼均用。经大鼠尾静脉注入孟加拉红3 min后,采用波长532 nm的绿激光,对距离视乳头1～3 PD内的视网膜静脉段进行激光照射,形成一段约1 PD的静脉血栓模型。随后眼底和视网膜荧光血管造影(fundus fluorescein angiography,FFA)连续3周观察模型的建立情况。并用光镜和透射电镜观察视网膜组织和血管结构。结果用激光联合孟加拉红的方法成功建立SD大鼠视网膜静脉阻塞的模型。FFA观察可见血流中断,远端血管纡曲扩张、视网膜出血等。光镜显示视网膜组织各层结构基本正常以及血管完全阻塞。电镜观察证实血管完全阻塞是由于血栓的形成。结论利用激光联合孟加拉红的方法可以成功建立大鼠视网膜静脉阻塞的模型。此方法对视网膜组织结构无明显损伤,为下一步研究视网膜静脉阻塞的治疗奠定了实验基础。Objective To develop experimental retinal vein occlusion with photodynamic method in combination with rose bengal dye in SD rats, and investigate the morphologic and histologic changes in the retina. Methods After SD rats tail intravenous injection of rose bengal,the rats retinal veins near the disc of 1-3 PD were exposed to diode laser（wave-length 532 nm） and formed complete vascular occlusion and the 1 PD thrombus could be seen. The eyes were observed with eye fundus check and fundus fluorescein angiography（FFA） every other day for three weeks. Then the eyes were enucleated and prepared for light and electron microscope examination. Results Retinal vein occlusion was developed successfully by combining photodynamic thrombosis and rose bengal dye in SD rats.Histopathologic features were consistent with changes of retinal vein occlusion. Furthermore,formation of retinal vein thrombus was reliable. Conclusions Photodynamic method combined with rose bengal dye is a simple,reliable and definitive experimental technique to produce rats retinal vein occlusion.

关 键 词：激光 光动力疗法 视网膜静脉阻塞 模型 

分 类 号：R454.3[医药卫生—治疗学] R774.1[医药卫生—临床医学]