新生猪缺氧缺血脑损伤模型纹状体基本病理特点及多巴胺能机制的初步研究  

Study of striatal dopaminergic and glutamatergic parameters in newborn piglets model of hypoxic-ischemic brain injury

作　　者：张妍芬[1,2] 王晓明[1] 郭启勇[1] 

机构地区：[1]中国医科大学附属盛京医院放射科,辽宁沈阳110004 [2]齐齐哈尔医学院附属五院大庆龙南医院磁共振影像科

出　　处：《实用放射学杂志》2014年第3期495-498,共4页Journal of Practical Radiology

基　　金：国家自然科学基金项目（30770632,30570541）.

摘　　要：目的 观察新生猪缺氧缺血性脑损伤纹状体多巴胺受体和谷氨酸的变化，初步探讨纹状体多巴胺能和谷氨酸能系统的相互作用机制。方法 使用新生猪缺氧缺血脑损伤模型，通过磁共振波谱（MRS）检测纹状体损伤时谷氨酸的代谢变化，以及用免疫组化法观察纹状体损伤时多巴胺D2受体和多巴胺转运蛋白（DAT）的改变，并进一步分析三者的相关性。结果 缺氧缺血后纹状体D2受体阳性神经元的数量随着时间延长始终在减少，其减少的区域恰好与基底节损伤区域一致，减少的程度也与基底节损伤程度相平行；神经元DAT阳性表达先增加后减少，缺氧缺血后12 h表达最强；Glx/Cr比值在缺氧缺血后早期增加并与DAT改变呈正相关（r=0.77）。结论 多巴胺能和谷氨酸能系统参与了由于缺氧缺血引起的纹状体损伤，且二者之间可能存在一定的交互作用。Objective To investigate changes of striatal dopamine receptor and glutamate following hypoxic-ischemic（HI） brain injury in newborn piglets, and to preliminary evaluate the interaction mechanism between the glutamatergic and dopaminergic systems. Methods Using the newborn piglets model of HI brain injury, glutamate metabolic changes were detect by magnetic resonance spectroscopy （MRS）, as well as dopamine D2 receptors （D2R） and dopamine transporter （DAT） changes were observed by immu- nohistochemistry, and the three correlations were further analyzed. Results After HI, striatal D2R positive neurons decreased. The areas of decreased positive neurons corresponding to the damaged regions of the striatum observed by HE staining, and the decreases were consistent with the degree of striatum. The DAT expression was up-regulated firstly followed by a down-regulation following HI brain insults in newborn piglets. Furthermore, the Glx/Cr ratio increased followed by a gradual decrease and there was a positive correlation between the concentration of glutamate and DAT expression （r = 0. 77）. Conclusion Glutamatergic and dopaminergic function are interdependent and act synergistically following HI brain insults in newborn piglets.

关 键 词：多巴胺转运蛋白 多巴胺受体 谷氨酸 缺氧缺血性脑病 新生猪 

分 类 号：R651.15[医药卫生—外科学] R-332[医药卫生—临床医学]