电针对去卵巢大鼠模型肠黏膜维生素D受体mRNA和蛋白表达的影响  

Effect of electroacupuncture on mRNA and protein expression of vitamin D receptor in intestinal mucosain ovariectomized rats

作　　者：欧阳钢[1] 唐曦[2] 莫非[1] 葛伟[1] 侯立皓[1] 娄青林[1] 顾刘宝[1] 

机构地区：[1]江苏省省级机关医院康复医学科,南京210024 [2]重庆医科大学附属儿童医院康复医学科

出　　处：《中华老年医学杂志》2014年第3期302-305,共4页Chinese Journal of Geriatrics

基　　金：国家自然科学基金资助项目

摘　　要：目的观察电针对去卵巢大鼠模型肠黏膜维生素D受体（VDR）mRNA和蛋白表达的影响，探讨针灸治疗绝经后骨质疏松症可能机制。方法60只3月龄SD健康雌性大鼠，随机分为手术组和假手术组，3个月造模成功后将手术组随机分为：模型组、假电针组、电针组、药物组。干预治疗12周后，测定腰椎及股骨骨密度，然后处死大鼠，提取小肠黏膜，采用荧光实时定量PCR和Western blot检测VDRmRNA和蛋白表达。结果成功复制绝经后骨质疏松症模型，经电针组和药物组干预治疗12周后，其大鼠骨密度与模型组相比均有明显提高，荧光实时定量PCR和Western blot检测结果显示电针组和药物组大鼠小肠黏膜VDRmRNA和蛋白表达上调，但电针组上调VDRmRNA的水平不及药物组。结论针灸治疗绝经后骨质疏松症机制之一可能与上调小肠黏膜VDRmRNA和蛋白表达量，增强了维生素D的生物活性有关。Objective To observe the effect of eleetroaeupuneture （EA） on intestinal vitamin D receptor （VDR） mRNA and protein expression in ovariectomized rat model, and to explore the possible mechanism of acupuncture treatment for postmenopausal osteoporosis. Methods Sixty 3- month-old female SD rats were randomly divided into operation group and sham group. 3 months after osteoporosis model was successfully produced, rats in operation group were randomly divided into model group, EA sham group, EA group and drug group. 12 weeks after intervention, bone mineral density （BMD） ol lumbar spine and lemur were detected, then rats were all sacrificed to extract the small intestinal mucosa. VDR mRNA and protein expressions were determined by real-time quantitative PCR and Western blotting. Results The model o{ postmenopausal osteoporosis was successfully established. The bone mineral density was significantly improved in EA group and drug group as compared with the model group at 12 weeks alter intervention. Western blotting and real- time quantitative PCR showed that small intestinal VDR mRNA and protein expressions were increased in EA group and drug group, but the increment of VDR mRNA expression was less in EA group than in drug group. Conclusions One of the mechanisms of acupuncture treatment for postmenopausal osteoporosis may be related with increasing the intestinal VDR mRNA and protein expression levels and enhancing the bioactivitv of vitamin D.

关 键 词：骨质疏松 绝经后 电针 维生素D 

分 类 号：R245[医药卫生—针灸推拿学]