适体核酶型人工核糖开关的设计  

The Artificial Aptazyme Based Riboswitch

作　　者：王佳稳 冯婧娴 林俊生[1,2] 刁勇[1,2] 

机构地区：[1]华侨大学分子药物研究院,泉州362021 [2]华侨大学生物医学学院,泉州362021

出　　处：《中国生物工程杂志》2014年第2期59-64,共6页China Biotechnology

基　　金：国家自然科学基金(81271691;81371669);国际科技合作项目(2011DFG33320);福建省科技重大项目(2013N5007)资助项目

摘　　要：适体核酶型核糖开关是近年出现的一种人工基因调控开关。最常见的适体核酶由锤头状核酶和适体组成,结构清晰,易于设计。作为一种顺式作用元件,适体核酶型核糖开关在特异性配体的作用下,无需蛋白质辅助,即可通过调节自身裂解反应,调控mRNA的翻译,可应用于多种细胞的基因调控。目前适体核酶型核糖开关的设计,主要是通过合理组装核酶与适体元件,整合到mRNA后再进行功能筛选。该基因调控开关调节幅度大、响应迅速、调控方式简洁,有可能应用于体内传感器、基因治疗、生物处理器等多个领域。The artificial aptazyme based riboswitch has emerged as a powerful tool for regulating gene expression in recent years. The most common aptazyme consists of a hammerhead ribozyme and an aptamer, it is simply a piece of structured RNA and easy to design. As a cis-acting element, aptazyme riboswitch can recognize specific targets and exert effective influence on translation without the help of protein co-factor through irreversible self-cleavage in vivo, which makes it a versatile platform functions in various cells. The most popular approach to generate an aptazme based riboswitch is to rationally cojoin a ribozyme to an aptamer and screen with a high throughout method after integrated into mRNA. With considerable magnitude of on-off control and rapid induction as well as concise regulation, this riboswitch will have important application in in vivo sensing, gene therapy and biological processor.

关 键 词：适体 模块组装 基因调控 锤头状核酶 

分 类 号：Q789[生物学—分子生物学]