PKH26标记骨髓基质干细胞经肋间动脉移植促进家兔脊髓损伤后神经功能恢复的研究  

Transplantation of PKH26-1abeled bone mesenchymai stem cells via intercostal arteries to promoteneurological function recovery in rabbits with spinal cord injury

作　　者：王晶[1] 李东卿[2] 王业杨[1] 蔡颖谦[3] 李超[1] 孙鸿涛[1] 张振兴[1] 李贵涛[1] 

机构地区：[1]广州,南方医科大学附属南粤医院广东省第二人民医院骨科,510317 [2]南方医科大学附属中山博爱医院骨科,510317 [3]南方医科大学珠江医院神经外科,510317

出　　处：《中华创伤骨科杂志》2014年第3期249-253,共5页Chinese Journal of Orthopaedic Trauma

基　　金：广东省科技汁划项目（20128031800266）;广东省医学科研基金（A2012138）

摘　　要：目的探讨PKH26标记骨髓基质干细胞（BMSCs）经肋间动脉移植对家兔脊髓损伤（SCI）后神经功能恢复的影响。方法采用动脉瘤央法制备家兔T9节段SCI模型，建模成功后随机分为损伤对照组和BMSCs移植组。损伤后7d，损伤对照组仅造模，不行处理；BMSCs移植组经肋间动脉注射0．5mL标记PKH26的BMSCs。损伤前、后行BBB后肢运动功能评分和神经电生理检测，损伤后2ld处死动物行脊髓组织病理学检查。结果损伤后7d移植前两组BBB评分差异无统计学意义（P〉0．05），损伤后14、21dBMSCs移植组BBB评分较损伤对照组高，差异有统计学意义（P〈0．05）。损伤后14、21dBMSCs移植组家兔的运动诱发电位（MEP）和感觉诱发电位（SEP）恢复较损伤对照组好，座异有统计学意义（P〈0．05）。损伤后21d在移植BMSCs家兔脊髓损伤区可发现PKH26标记的BMSCs阳性细胞聚集，苏木素-伊红染色显示两组脊髓损伤区均町见胶质瘢痕和夺洞形成，但BMSCs移植组空洞面积小于损伤对照组；BMSCs移植组脊髓损伤组织内神经微丝200阳性表达强于损伤对照组，差异有统计学意义（P〈0．05）。结论PKH26标记BMSCs经肋间动脉移植可有效促进家兔SCI后神经功能的恢复。Objective To investigate the effect of transplantation of PKH26-1abeled bone mes- enchymal stem cells （BMSCs） via intercostal arteries on the neurological function recovery in rabbits with spinal cord injury （SCI） . Methods The SCI models were made by aneurysm clips at the level of T9 segment in 20 rabbits. The successful rabbit SCI models were randomly divided into 2 equal groups. Seven days after SCI, tbe control group was not treated while in the transplantation group 0. 5 mL of PKH26-1abeled BMSCs was injected through intercostal arteries. Basso-Beattie-Bresnahan （BBB） scoring and neuro-electrophysiology detection were conducted before and after SCI. The animals were sacrificed to harvest spinal cord spec.imens for histopathological examination 21 day＇s after SCI. Results There were no significant differences between the 2 groups in BBB score 7 days after SCI （ P 〉 0.05） . At 14 and 21 days after SCI, the BBB scores were significantly higher and recovery of motor evoked po-tentials （MEP） and somatosensory evoked potentials （SEP） was significantly better in the transplantation group than in the control group （P 〈 0.05） . PKH26-positive BMSCs gathered at the injured spinal sites 21 days after SCI in the transplantation group. Hematoxylin-Eosin （HE） staining showed visible glial scar tissue and cavity formation at the injured spinal sites in the 2 groups 21 days after SCI, but the cavity area in the transplantation group was smaller than in the control group. NF 200 positive expression was significantly enhanced in the transplantation group than in the control group （ P 〈 0.05） . Conclusion Transplantation of PKH26-1abeled BMSCs via intercostal arteries can effectively promote the neurological function recovery in rabbits with spinal cord injury.

关 键 词：骨髓 间质干细胞移植 脊髓损伤 肋间动脉 PKH26 

分 类 号：R651.2[医药卫生—外科学]