北京地区儿童急性上呼吸道感染中肠道病毒感染谱的分析  

Spectrum analysis of enteroviruses in children with acute respiratory tract infections in Beijing

作　　者：李霖琳 谢正德[2] 刘春艳[2] 相子春[1] 任丽丽[1] 申昆玲[2] 王健伟[1] 

机构地区：[1]中国医学科学院北京协和医学院病原生物学研究所,北京100730 [2]首都医科大学附属北京儿童医院北京市儿科研究所儿科学国家重点学科省部共建儿科重大疾病研究重点实验室,北京100045

出　　处：《基础医学与临床》2014年第4期485-489,共5页Basic and Clinical Medicine

基　　金：国家科技厦大专项"传染病监测技术平台"项目(2012ZX10004-206)

摘　　要：目的了解北京地区儿童急性上呼吸道感染中肠道病毒(EV)的感染谱。方法收集2009-05-09于北京儿童医院就诊的急性上呼吸道感染患者的咽拭子1 121份,用反转录-聚合酶链反应(RT-PCR)方法对EV的VP1基因进行了扩增并测序。结果发现55例为EV阳性,分属14个血清型。其中44例属于A种EV(EV-A),11例为EV-B。检出EV与手足口病及无菌性脑膜炎中检测到的EV具有高度同源性,但和EV原型株相比较均产生了不同程度的变异。结论2009年北京地区急性上吸道感染儿童中以EV-A感染为主,与成人病原谱存在差异。Objective To determine the spectrum of enteroviruses （EV） in children with acute respiratory tract in- fections （ARTI） in Beijing. Methods Throat swabs were collected from 1,121 children at the time of admission to the Beijing Children＇s Hospital for ARTI during May 2009 through September 2009. To detect EV, the viral protein 1 （ VP1 ） gene were amplified by reverse transcription PCR and verified the findings by sequence analysis. Results EV was detected in 55 children and 14 EV serotypes were identified. Among of them, 7 EV serotypes including 44 cases belonged to EV-A and the others belonged to EV-B. The sequences of EV in this research had very high homology with that detected in hand-foot-and-mouth disease or aseptic meningitis. Compared to prototype, muta- tions were happened in clinical strains. Conclusions EV-A was dominant in children with acute respiratory tract infections which was different to that of the adult in Beijing in 2009.

关 键 词：肠道病毒 呼吸道感染 感染谱 

分 类 号：R373.2[医药卫生—病原生物学]