木犀草素对急性免疫性肝损伤小鼠的保护作用  

Protective effects of luteolin on immune-mediated liver injury in mice

作　　者：张琦[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院检验科,上海200011

出　　处：《上海交通大学学报（医学版）》2014年第3期329-332,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(81200299)~~

摘　　要：目的探讨木犀草素对免疫介导的肝损伤的保护作用。方法 C57BL/6小鼠随机分为实验组和对照组(n=5),利用刀豆蛋白A(ConA)尾静脉注射诱导建立免疫性肝损伤小鼠模型。实验组小鼠建模前经腹腔注射木犀草素预处理。全自动生化分析仪检测血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平;苏木精-伊红染色(HE染色)光学显微镜观察小鼠肝组织学改变,TUNEL检测分析细胞凋亡;酶联免疫吸附试验(ELISA)检测血清肿瘤坏死因子-α(TNF-α)和γ干扰素(IFN-γ)水平。结果实验组小鼠血清ALT和AST水平显著低于对照组(P<0.001);与对照组比较,实验组小鼠肝脏炎症细胞浸润情况较轻,肝细胞发生凋亡较少;实验组小鼠血清TNF-α和IFN-γ水平显著低于对照组(P<0.05和P<0.01)。结论木犀草素预处理能够降低ConA诱导的炎症介质的分泌,减轻小鼠免疫性肝损伤。Objective To investigate the protective effects of luteolin on immune-mediated liver injury in mice. Methods The mouse model of immune-mediated liver injury was established by ConA injection to C57BL/6 mouse. Serum ALT and AST levels in mice were determined by an automatic biochemistry analyzer. HE stain and TUNEL stain were used to determine the extent of liver injury. Serum TNF-a and IFN-γ concentrations were measured by ELISA. Results Luteolin treatment significantly decreased the ALT and AST levels in ConA-induced hepatitis （CIH） mice （P 〈 0.001）. Luteolin treated mice also displayed less inflammatory infiltration in the liver and hepatocyte apoptosis. Meanwhile, luteolin significantly inhibited the release of inflammatory cytokines such as TNF-a and IFN-γ in ConA injected mice （P 〈0.05, P 〈 0.01）. Conclusion Luteolin can protect mice from immune-mediated liver injury by inhibiting the release of ConA-induced inflammation mediator.

关 键 词：木犀草素 刀豆蛋白A 免疫介导的肝损伤 炎症介质 

分 类 号：R285.5[医药卫生—中药学]