卵母细胞分泌的生长分化因子-9和骨形成蛋白-15与卵母细胞发育潜能的关系  

Relationship of Growth Differentiation Factor-9 and Bone Morphogenetic Protein-15 Secreted by Oocytes with Oocyte Development Potency

作　　者：常秀峰[1] 张敏[1] 吴小华 徐清华[1] 朱爱萍[1] 包莉莉[1] 于晓惠[1] 

机构地区：[1]白求恩国际和平医院妇产科生殖医学中心,石家庄050082

出　　处：《生殖与避孕》2014年第3期200-204,共5页Reproduction and Contraception

摘　　要：目的:探讨卵母细胞分泌的生长分化因子-9(GDF-9)和骨形成蛋白-15(BMP-15)与卵母细胞发育潜能的关系。方法:收集因输卵管性不孕接受IVF-ET治疗患者的卵泡,每侧卵巢穿刺取卵时首先选择一个直径≥18 mm卵泡抽吸,对卵母细胞进行成熟度分型,并观察其受精、卵裂及胚胎评分情况。受精后按是否形成优质胚胎将将卵泡分为优质卵母细胞组和非优质卵母细胞组,采用Western blotting方法检测比较各组卵泡液中GDF-9和BMP-15的含量。结果:①优质卵母细胞组的GDF-9蛋白相对含量为2.498±0.133,非优质卵母细胞组为1.305±0.406,差异有统计学意义(P=0.008)。②优质卵母细胞组的BMP-15蛋白相对含量为3.379±0.482,非优质卵母细胞组为1.336±0.214,差异有统计学意义(P=0.003)。结论:在优质卵母细胞组卵泡液中GDF-9和BMP-15水平均较高,提示人卵泡液中GDF-9、BMP-15水平可以反映卵母细胞的发育潜能,GDF-9、BMP-15水平可以作为评价卵母细胞质量的指标。Objective： To evaluate the relationship of growth differentiation factor-9 （GDF-9） and bone morphogenetic protein-15 （BMP-15） secreted by oocytes with oocyte development potential. Methods： Follicle fluid （FF） from the first aspirated follicle （diameter 〉/18 mm） of each ovary was carefully collected in a tube. Individual oocytes were inseminated and subsequently assessed on the basis of their maturation, fertilization, cleavage and preimplantation development. The occytes were divided into good-quality oocyte group which developed to good- quality embryos and bad-quality oocyte group. FF samples were centrifuged and collected. The GDF-9 and BMP- 15 levels in FF were measured by Western blotting. Results： 1） The level of GDF-9 in FF was 2.498 ___ 0.133 in good-quality oocyte group and 1.305 ___ 0.406 in bad-quality oocyte group. The level of GDF-9 in good-quality oocyte group was significantly higher than that in bad-quality oocyte group （P=0.008）. 2） The level of BMP- 15 in FF was 3.379 _ 0.482 in good-quality oocyte group and 1.336 -t- 0.214 in bad-quality oocyte group. The level of BMP-15 in good-quality oocyte group was significantly higher than that in bad-quality oocyte group （P= 0.003）. Conclusion： The level of GDF-9 and BMP-15 in FF appears to be potential factors in predicting oocyte quality and subsequent embryo development.

关 键 词：卵母细胞分泌因子(OSFs) 生长分化因子-9(GDF-9) 骨形成蛋白-15(BMP-15) 卵母细胞发育潜能 

分 类 号：R711.6[医药卫生—妇产科学]