针刺胃扩张模型大鼠内关、足三里等穴位下丘脑室旁核相关神经元的反应  

Response of gastric-related neurons in the hypothalamic paraventricular nucleus to acupuncture at Neiguan and Zusanli in a rat model of gastric distension

作　　者：陈姝[1] 雍春燕 陈恒[1] 初晓[1] 张超[1] 谭程[1] 叶兰[1] 李江山[1] 

机构地区：[1]湖南中医药大学针灸推拿学院,湖南省长沙市410007

出　　处：《中国组织工程研究》2014年第5期675-680,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金面上项目课题(81273677)~~

摘　　要：背景:既往有关针刺对胃功能调节的神经机制研究多以外周神经为主,涉及中枢,尤其是高位中枢的研究较少。目的:探讨针刺"内关"、"足三里"等穴位对下丘脑室旁核胃相关神经元作用的影响。方法:运用微电极细胞外记录技术,在大鼠胃扩张的基础上,找出室旁核胃相关神经元,予以手针刺激"内关"、"足三里"等穴位各30 s,观察其对室旁核胃相关神经元的影响。结果与结论:60只大鼠共记录到109个下丘脑室旁核神经元放电,109个下丘脑室旁核神经元中与胃扩张相关的神经元有56个,56个胃扩张相关神经元中,对针刺足三里、内关、脾俞、胃俞有反应的神经元个数分别是44,47,29,33,有反应的神经元出现的比例分别是78.57%,83.47%,51.79%,58.93%。结果表明下丘脑室旁核中存在同时对胃扩张刺激和针刺刺激起反应的躯体内脏汇聚神经元,且参与针刺对胃功能的调节作用。BACKGROUND：Previous studies concerning neuromechanism of acupuncture for regulating gastric function mainly focused on peripheral nerve, involving in central nervous system. In particular, there are few studies addressing higher central nervous system.OBJECTIVE：To explore the effects of acupuncture at Neiguan （PC6） and Zusanli （ST36） on gastric-related neurons in the hypothalamic paraventricular nucleus.METHODS：Using microelectrode extracellular recording technique, gastric related neurons in the paraventricular nucleus were found in gastric distension rats. Stimulation was conducted for 30 seconds by hand acupuncture at Neiguan and Zusanli. The effects of acupuncture on gastric-related neurons in the paraventricular nucleus were observed. RESULTS AND CONCLUSION：The discharge of 109 neurons in the hypothalamic paraventricular nucleus of 60 rats was recorded. A total of 56 gastric-related neurons of the 109 neurons were observed. The number of neurons response to acupunctures at Zusanli, Neiguan, Pishu （BL20） and Weishu （BL21） were respectively 44, 47, 29, and 33, with the reaction proportion of 78.57%, 83.47%, 51.79%and 58.93%. Results suggested that there exist gastric distension stimulation and acupuncture stimulations from neurons soma to visceral reaction in the hypothalamic paraventricular nucleus, which simultaneously participates in the regulation of acupuncture on the gastric function.

关 键 词：实验动物 组织构建 下丘脑室旁核 胃相关神经元 针刺刺激 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]