温阳益髓中药干预兔膝骨关节炎软骨基质金属蛋白酶的表达  

Effects of warming the yang and benefiting the marrow on the expression of matrix metalloproteinases in articular cartilage of rabbits with knee osteoarthritis

作　　者：单鹏程[1] 何名江[1] 张洪美[1] 荆琳[1] 张淳[1] 张仁卓[1] 闫奇[1] 沙益辉 

机构地区：[1]中国中医科学院望京医院骨关节一科,北京市100102

出　　处：《中国组织工程研究》2014年第7期997-1002,共6页Chinese Journal of Tissue Engineering Research

基　　金：北京市自然科学基金(7102166)~~

摘　　要：背景:目前临床上关于温阳益髓中药治疗膝骨关节炎对软骨基质金属蛋白酶表达影响的研究还较少有报道。目的:制作兔膝骨关节炎模型观察温阳益髓中药对软骨基质金属蛋白酶表达的影响。方法:健康成年新西兰大白兔96只,随机选取72只采用石膏外固定方法制作兔膝骨关节炎模型。确定造模成功后再随机分为3组,模型组不做处理;中药治疗组每日灌胃方药提取液24 mL/kg,药物对照组每日灌胃葡立胶囊(盐酸氨基葡萄糖)24 mg/kg,1次/d,至造模成功后8周。另外24只新西兰大白兔作为空白对照。结果与结论:PCR方法定量分析骨关节炎模型组软骨组织中基质金属蛋白酶1、基质金属蛋白酶3、基质金属蛋白酶13表达水平均显著高于其他3组。中药治疗组及药物对照组中基质金属蛋白酶1、基质金属蛋白酶3及基质金属蛋白酶13的表达较模型组明显降低。说明温阳益髓中药治疗兔膝骨关节炎能够有效抑制兔骨关节炎软骨基质金属蛋白酶的表达。BACKGROUND：There are few reports concerning effects of warming the yang and benefiting the marrow for the knee osteoarthritis on the expression of matrix metal oproteinase. OBJECTIVE：To observe the effect of warming the yang and benefiting the marrow on the expression of matrix metal oproteinase in rabbit models of knee osteoarthritis. METHODS：Of 96 healthy adult New Zealand rabbits, 72 rabbits were randomly selected for making rabbit models of knee osteoarthritis using plaster external fixation. After success model establishment, the rabbits were randomly assigned to three groups. Model group was left intact. Chinese medicine group received daily intragastric administration of drug extract 24 mL/kg. Drug control group was daily intragastrical y administered Puli Capsule （glucosamine hydrochloride） 24 mg/kg, once a day, until the eighth week of success model induction. An additional 24 New Zealand rabbits served as blank controls. RESULTS AND CONCLUSION：Using quantitative PCR, matrix metal oproteinase-1, matrix metal oproteinase-3 and matrix metal oproteinase-13 expression was significantly higher in the model group than that in the other three groups. Matrix metal oproteinase-1, matrix metal oproteinase-3 and matrix metal oproteinase-13 expression was significantly lower in the Chinese medicine group and drug control group than that in the model group. These results indicated that warming the yang and benefiting the marrow for knee osteoarthritis in rabbits could effectively inhibit the expression of matrix metal oproteinase in rabbits.

关 键 词：组织构建 软骨组织工程 温阳益髓 中药 骨关节炎 基质金属蛋白酶 软骨 盐酸氨基葡萄糖 北京市自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]