不同分期骨关节炎滑膜细胞中COX-2 mRNA表达的差异  

COX-2 mRNA expression at different stages of osteoarthritis synoviocytes

作　　者：曾明珠 段戡[2] 袁长深[2] 梅其杰[2] 秦凯[3] 

机构地区：[1]广西自治区骨伤医院,广西壮族自治区南宁市530012 [2]广西中医药大学第一附属医院,广西壮族自治区南宁市530023 [3]广西中医药大学研究生学院,广西壮族自治区南宁市530001

出　　处：《中国组织工程研究》2014年第7期1003-1008,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:COX-2基因实际存在于关节成纤维样滑膜细胞里,影响骨关节炎症的发生、发展。阐明不同分期骨关节炎滑膜细胞中COX-2基因表达量的差异对进一步深入了解骨关节炎的发生与发展,以及滑膜细胞在这一过程中的作用,具有重要的理论意义。目的:分析COX-2 mRNA在不同分期骨关节炎成纤维样滑膜细胞中表达的差异。方法:收集膝骨关节炎病变滑膜44例及正常滑膜12例,经原代细胞培养生长至4代的成纤维样滑膜细胞用于实验,应用实时荧光定量RT-PCR检测骨关节炎和正常成纤维样滑膜细胞中COX-2 mRNA的表达情况,最后使用2-ΔΔCt方法进行相对定量分析。结果与结论:COX-2 mRNA在骨关节炎的成纤维样滑膜细胞中的表达明显高于正常成纤维样滑膜细胞(P<0.05),其中骨关节炎早期组表达最高,与骨关节炎中期组、晚期组比较差异有显著性意义(P<0.05),骨关节炎中期组与晚期组中表达量差异无显著性意义(P>0.05)。结果说明COX-2mRNA可能是骨关节炎症病变过程中的重要生物学指标,且主要起作用于骨关节炎病变早期。BACKGROUND：COX-2 gene actual y exists in the joint fibroblast-like synoviocytes, it affects osteoarthritis occurrence and development. Understanding the differences of COX-2 gene expression levels at different stages of osteoarthritis synoviocytes has important theoretical significance for the occurrence and development of osteoarthritis, as wel as the role of synoviocytes in this process. OBJECTIVE：To analyze the difference of COX-2 mRNA at different stages of osteoarthritis fibroblast-like synoviocytes. METHODS：Synovial membrane from 44 osteoarthritis patients and 12 normal cases were selected. Primary cells were cultured to passage 4 fibroblast-like synoviocytes for the use in the experiment. COX-2 mRNA expression in osteoarthritis fibroblast-like synoviocytes and normal fibroblast-like synoviocytes was detected using real-time fluorescence quantitative RT-PCR. The relative quantitative analysis was performed using 2-ΔΔCt method. RESULTS AND CONCLUSION：Expression of COX-2 mRNA in osteoarthritis fibroblast-like synoviocytes was significantly higher than that in normal fibroblast-like synoviocytes （P0.05）. COX-2 mRNA might be important biological marker for the inflammation in osteoarthritis, and mainly plays a role in early osteoarthritis stage.

关 键 词：组织构建 软骨组织工程 实时荧光定量RT-PCR 骨关节炎 成纤维样滑膜细胞 COX-2MRNA 

分 类 号：R318[医药卫生—生物医学工程]