骨髓间充质干细胞条件培养液对瘢痕成纤维细胞生物活性的影响  

Effects of bone marrow mesenchymal stem cell conditioned medium on bioactivity of scar fibroblasts

作　　者：武艳[1] 张春雷[1] 刘阳[1] 李洪志[1] 于晶[1] 包海花[1] 郭冉[1] 袁晓环[1] 

机构地区：[1]牡丹江医学院,黑龙江省牡丹江市157011

出　　处：《中国组织工程研究》2014年第7期1009-1014,共6页Chinese Journal of Tissue Engineering Research

基　　金：黑龙江省卫生厅科研课题(2011-291);牡丹江医学院科学技术研究项目(ZS201302)~~

摘　　要：背景:间充质干细胞移植可通过多种调节机制促进皮肤创伤修复,抑制瘢痕形成,目前多数学者认为间充质干细胞分泌的生物活性因子起主要作用。目的:观察骨髓间充质干细胞条件培养液对增生性瘢痕成纤维细胞增殖能力及胶原合成的影响。方法:分离培养人骨髓间充质干细胞和增生性瘢痕成纤维细胞,制备骨髓间充质干细胞条件培养液。将12,24,48 h收集的条件培养液孵育体外培养的增生性瘢痕成纤维细胞24 h,并与空白对照组比较,采用CCK-8实验检测增生性瘢痕成纤维细胞的增殖活性,Real-time PCR检测增生性瘢痕成纤维细胞中Ⅰ型和Ⅲ型胶原的表达。结果与结论:与空白对照组相比较,在24 h和48 h收集的骨髓间充质干细胞条件培养液对增生性瘢痕成纤维细胞的增殖具有明显的抑制作用(P<0.01),对其胶原的合成也具有显著的抑制作用(P<0.01)。结果表明骨髓间充质干细胞条件培养液可通过分泌抗纤维化的生物活性因子抑制增生性瘢痕成纤维细胞的增殖和胶原产生,为细胞疗法策略减轻瘢痕提供了新的理论支持。BACKGROUND：Mesenchymal stem celltransplantation promoted skin repair in trauma via various regulatory mechanisms and inhibited scar formation. At present, many scholars believed that bioactive factors secreted by mesenchymal stem cells played an important role. OBJECTIVE：To investigate the effects of bone marrow mesenchymal stem cellconditioned medium on the proliferation and col agen synthesis of hypertrophic scar fibroblasts. METHODS：Human bone marrow mesenchymal stem cells and hypertrophic scar fibroblasts were isolated and cultured, and bone marrow mesenchymal stem cellconditioned medium was prepared. Hypertrophic scar fibroblasts were cultured in vitro with 12, 24, and 48 hour-col ected conditioned medium for 24 hours, which was compared with blank control group. The proliferation of cells was determined by CCK-8. Type I and type III col agen expression in hypertrophic scar fibroblasts was detected using real-time PCR. RESULTS AND CONCLUSION：Compared with the blank control group, 24 and 48 hour-col ected conditioned medium significantly inhibited the proliferation of hypertrophic scar fibroblasts （P〈0.01）, and also suppressed col agen synthesis of hypertrophic scar fibroblasts （P〈0.01）. Results suggested that bone marrow mesenchymal stem cellconditioned medium inhibited the proliferation and col agen synthesis of hypertrophic scar fibroblasts by secreting anti-fibrotic bioactive factors, which may provide new theoretical supports for celltherapy to reduce cutaneous scarring.

关 键 词：组织构建 成纤维细胞 骨髓间充质干细胞 增生性瘢痕 瘢痕成纤维细胞 生物活性因子 细胞治疗 

分 类 号：R318[医药卫生—生物医学工程]