KH-550/PPy/PET单丝制备及生物相容性评价  

Preparation and biocompatibility of gamma-aminopropyl triethoxysilane/polypyrrole/ polyester monofilament

作　　者：王莹[1] 陈莹[2] 岳秉飞[1,3] 唐劲天[1,2] 李莹[1] 张小娜[1] 

机构地区：[1]北京中医药大学中药学院生物制药系,北京市100102 [2]清华大学工程物理系医学物理与工程研究所,北京市100084 [3]中国食品药品检定研究院实验动物资源研究所,北京市100050

出　　处：《中国组织工程研究》2014年第8期1244-1249,共6页Chinese Journal of Tissue Engineering Research

基　　金：中国博士后科学基金第53批面上项目(2013M530637)~~

摘　　要：背景:目前关于移植毛发的研究大部分是以患者本身拥有足够的毛发为基础,对于治疗大面积的秃发没有明显的效果,而植入性人造头发可解决这一问题。目的:制备硅烷偶联剂γ-氨基丙基三乙氧基硅烷/聚吡咯膜/涤纶(KH-550/PPy/PET)单丝复合材料作为植入性人造头发,并对制备效果进行评定,测定复合材料对小鼠成纤维细胞L929的细胞毒性。方法:涤纶单丝经过去污处理、碱减量处理、硅烷偶联剂处理和聚吡咯镀膜等一系列过程制备成KH-550/PPy/PET复合材料。采用直接接触法,将此复合材料和L929细胞共孵育。采用CCK-8法于培养第1,2,3,5,7天进行细胞毒性检测。结果与结论:制备所得的复合材料表面光滑,聚吡咯镀膜完整,无断裂,且耐磨性能良好,不易脱落。从扫描电子显微镜、傅里叶变换红外分光检测减量处理后的涤纶单丝表面聚吡咯的镀膜量明显高于未经过碱减量处理的涤纶单丝。复合材料和L929细胞共孵育,培养第1,2,3,5,7天细胞存活率依次为100%,80.37%,73.26%,81.96%,77.50%,细胞毒级为1级。结果表明,硅烷偶联剂γ-氨基丙基三乙氧基硅烷的引入有效地提高了聚吡咯膜与涤纶单丝的结合力,实验所制备的硅烷偶联剂γ-氨基丙基三乙氧基硅烷/聚吡咯膜/涤纶复合材料生物相容性较好,无明显细胞毒性。BACKGROUND：Now most of the researches on transplant hair are based on the patients with enough hair, but there is no apparent corrective effect for large area of alopecia. Implanted artificial hairs can solve this problem. OBJECTIVE：To make and evaluate the γ-aminopropyl triethoxysilane/polypyrrole/polyester （KH-550/PPy/PET） composite monofilament as implanted artificial hairs, and to carry out cytotoxicity tests using NCTC clone 929 cels with composite monofilament. METHODS：KH-550/PPy/PET composite monofilament was prepared in a series of steps, including pre-spotting, alkali treatment, silane coupling agent treatment and polypyrrole coating. The viability of NCTC clone 929 cels were detected after 1, 2, 3, 5, 7 days of co-culture with composite monofilament by using cellcounting kit-8. RESULTS AND CONCLUSION：KH-550/PPy/PET composite monofilament had a smooth surface without crack. The PPy films did not come off accidentaly and had good wearability. After alkali treatment, PPy quality on the surface of monofilament was significantly heavier than before. Using silane coupling agent （KH-550） could＆nbsp;effectively enhance biocompatibility and binding force of polyester monofilaments. After co-cultured with composite monofilaments, the viability of NCTC clone 929 cels was 100%, 80.37%, 73.26%, 81.96%, 77.50% at days 1, 2, 3, 5, 7 respectively. The level of cytotoxicity was grade 1. The results show that KH-550 can effectively enhance the binding force between PPy and PET monofilament, and the prepared KH-550/PPy/PET composite monofilament has good biocompatibility and no acute toxicity.

关 键 词：生物材料 材料相容性 植入性人造头发 KH-550 聚吡咯 涤纶 

分 类 号：R318[医药卫生—生物医学工程]