穿心莲内酯对脑缺血大鼠认知功能的影响  

Effects of Andrographolide on Cognitive Function in Rats with Chronic Cereral Ischemia

作　　者：夏颖[1] 王成夭[1] 咸淑悦 李心怡[1] 马敏[1] 潘霞[1] 

机构地区：[1]武汉大学中南医院麻醉科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2014年第2期174-177,共4页Medical Journal of Wuhan University

基　　金：湖北省卫生厅资助项目(编号:JX4B23);湖北省自然科学基金资助项目(编号:ZRY1010)

摘　　要：目的:观察穿心莲内酯对慢性脑缺血大鼠认知功能的影响,探讨穿心莲内酯对脑的保护作用机制。方法:健康雄性SD大鼠48只,3-4月龄,体重250-300g,随机分成4组(n=12):假手术组(SH组)、慢性脑缺血组(IS组)、IS+二甲基亚砜组(DMSO组)和IS+穿心莲内酯组(AND组)。IS组、DMSO组和AND组采用永久结扎双侧颈总动脉法制备大鼠慢性缺血模型,DMSO组和AND组分别于双侧颈总动脉结扎后3,12,24,48h时腹腔注射0.2ml的5%DSMO和0.2ml含有10mg/kg穿心莲内酯的5%DSMO,IS组大鼠在术后相同时间点腹腔内注射等体积的生理盐水,SH仅穿线,不结扎。2周后进行Morris水迷宫测试认知能力;HE染色观察海马CA1区神经细胞形态,TUNEL染色法检测大鼠神经细胞凋亡,Western blotting检测海马组织Bcl-2蛋白的表达。结果:与SH比较,IS组和DMSO组于定位航行实验第2-5天、AND组于实验第2天逃避潜伏期延长,IS组、DMSO组、AND组第1象限停留时间缩短,细胞凋亡率增加,Bcl-2表达上调(P<0.05);与IS组比较,AND组于定位航行实验第3-5天逃避潜伏期缩短,第1象限停留时间延长,细胞凋亡率降低,Bcl-2表达上调(P<0.05);IS组与DMSO组间比较上述各指标差异无统计学意义(P>0.05)。结论:穿心莲内酯可改善慢性脑缺血大鼠认知功能,其机制可能与增加抗凋亡蛋白Bcl-2表达、减少神经细胞凋亡有关。Objective： To investigate the effect of andrographolide on cognitive function of rats with chronic cerebral ischemia and the protective effect of andrographolide on the brain against ischemia. Methods： Forty-eight male SD rats aged 3-4 months weighing 250-300 g were randomly divided into four groups （n： 12 each）： sham operation group （SH）, chronic cerebral ischemia （IS）, IS＋DMSO group （DMSO）, and IS＋ andrographolide group （AND）. The permanent occlusion of bilateral common carotid arteries in SD rats was adopted to set up the chronic fore-brain ischemia model. After two weeks, the cognitive function was assessed by Morris water maze （MWM）. The morphologic changes of CA1 region of hippocampus were observed with HE staining. The apoptosis was examined by method of TUNEL, and the expression of Bcl-2 protein in hippoeampus was detected by Western blotting. Results： Compared with group IS, the escape latencies in MWM of AND group were shorter （P〈0.05）. The rats of AND group spent moretime in the platform region than that of chronic cerebral ischemia rats in probe trials. The ischemia injury of the neurons in hippocampus CA1 of AND group was milder than that in IS group. The apoptotic rate of nerve cells in AND group was lower than that in IS group （P〈0.05）. The Bcl-2 protein expression in AND group was higher than that in IS group （P〈0.05）. In each detection index, there was no significant difference between IS and DMSO groups （P〉0. 05）. Conclusion： Andrographolide can improve the cognitive impairment after chronic cerebral hypoperfusion in rats, which can increase the expression of anti-apoptosis Bel-2 protein and restrain apoptosis of cranial nerve cells.

关 键 词：慢性脑缺血 认知 穿心莲内酯 抗凋亡 脑保护 

分 类 号：R614.2[医药卫生—麻醉学]