鞣花酸抑制黑素细胞黑素合成及黑素传递的实验研究  

Effects of Ellagic Acid on Melanogenesis and Melansome Transfer

作　　者：刘栋[1] 潘小钢[1] 李雅琳[1] 张峻岭[1] 马慧军[2] 

机构地区：[1]天津市中医药研究院附属医院皮肤科,300021 [2]解放军第309医院皮肤科,北京

出　　处：《天津医药》2014年第3期208-210,共3页Tianjin Medical Journal

基　　金：天津市卫生局科技基金(项目编号:06KY01);广州宝洁有限公司(P&G)科技基金

摘　　要：目的观察鞣花酸对黑素细胞(melanocyte,MC)合成黑素以及传递黑素的影响并探讨其机制。方法分别纯化培养来自人包皮的表皮黑素细胞和角质形成细胞(keratinocyte,KC),传第2代后以1∶10的比例将两细胞接种到3 cm×3 cm的小培养皿中,单独或混合培养的细胞经高、中、低(100、10、1 mg/L)3种浓度的鞣花酸干预48 h后,分别检测干预前后黑素细胞中黑素含量、酪氨酸酶活性的变化,采用流式法检测混合培养细胞中黑素传递的情况,以10 nmol/L熊果苷为阳性对照。结果 3种浓度鞣花酸浓度均可下调细胞酪氨酸酶活性,并呈浓度依赖性。除了1 mg/L的鞣花酸对黑素细胞黑素合成无明显作用,其余浓度的鞣花酸均可降低黑素含量。鞣花酸同时具有抑制黑素传递的作用。结论鞣花酸可抑制黑素合成及黑素传递,或可作为一种皮肤美白剂应用。Objective To investigate the effect of ellagic acid on human epidermal melanocyte melanogenesis and melanin transfer, and the mechanism thereof. Methods The human melanocytes and and keratinocytes were co-cultured and purified. After passing the second generation, cells of 1∶10 ratio were inoculated into the small dish （3 cm ＆#215; 3 cm）. The changes of melanin content and tyrosinase activity in melanocytes were detected before and after intervention with ellagic acid （100, 10 and 1 mg/L） for 48 h. The melanin transfer in cultured cells was detected by flow cytometry method. The 10 nmol/L arbutin was used as the positive control. Results The tyrosinase activity was down-regulated by ellagic acid in a dose-dependent manner. The ellagic acid can reduce the melanin content except for the 1 mg/L of ellagic acid. The melanin transfer was also inhibited by ellagic acid in a dose-dependence manner. Conclusion Ellagic acid can be used for skin-whitening cosmetic and the depigmenting effect might be due to the down-regulation of melanogenesis and melanin transfer.

关 键 词：鞣花酸 黑素细胞 一元酚单氧酶 角蛋白细胞 

分 类 号：R751[医药卫生—皮肤病学与性病学]