普伐他汀对大鼠急性心肌梗死后热休克蛋白B7表达的影响  

The Effect of Pravastatin on the Expression of HSPB7 in Acute Myocardial Infarction Rats

作　　者：蒋友旭 张丽华[1] 王小曼[1] 李盼盼[1] 

机构地区：[1]郑州大学第二附属医院心血管内科,450014

出　　处：《天津医药》2014年第3期228-230,I0007,共4页Tianjin Medical Journal

基　　金：河南省医学科技攻关计划项目(项目编号:201204029)

摘　　要：目的观察急性心肌梗死(AMI)大鼠梗死区心肌组织中热休克蛋白B7(HSPB7)mRNA和蛋白的表达水平,研究普伐他汀对HSPB7表达的干预作用。方法 80只AMI大鼠随机分为AMI组和普伐他汀(P)组,每组40只,同时设假手术(SH)组大鼠40只,再将3组大鼠分为1、3、6和12 h组,每个时间组10只。SH组开胸后不结扎冠脉;AMI组开胸后结扎冠状动脉左前降支;P组开胸后结扎冠状左前降支,同时给予普伐他汀0.5 mg/(kg·d)灌胃;其余组给予等量蒸馏水灌胃。分别于各时间点处死大鼠,取左心室梗死区心肌组织,SH组取对应部位的心肌组织,分别采用逆转录聚合酶链反应(RT-PCR)和免疫组化法检测HSPB7 mRNA和蛋白的表达。结果 AMI组和P组大鼠各时点梗死区心肌组织HSPB7 mRNA和蛋白表达均高于SH组(P<0.01);梗死后1 h,梗死区心肌HSPB7 mRNA和蛋白的表达增加,在3 h时达到高峰,6 h组、12 h组HSPB7 mRNA和蛋白的表达仍高于SH组。且P组中各时间点HSPB7的表达高于AMI组。结论 HSPB7能够在AMI早期表达,普伐他汀可促进AMI后梗死区心肌组织HSPB7表达,可能是其在心梗早期起到保护心肌作用的机制之一。Objective To observe the effect of pravastatin on the expression of heat shock protein B7 （HSPB7） in acute myocardial infarction （AMI） rats. Methods A total of 80 AMI rats were randomly divided into AMI group and pravas-tatin （P） group. Forty SD rats were used as sham operation （SH） group. Rats were then subdivided into 1 h, 3 h, 6 h and 12 h subgroups （10 for each group）. Rats were not ligated after thoracotomy in SH group. The 1eft anterior descending coronary ar-tery was ligated in rats of AMI group. The 1eft anterior descending coronary artery was ligated in P group and given intragas-tric administration of 0.5 mg/（kg ＆#183; d） pravastatin. The other groups were given the same amount of normal saline via gavage. The left ventricle infarcted myocardial tissues were taken at each time intervals. The corresponding myocardial tissues were harvested in sham-operated rats. The HSPB7 mRNA and protein expressions were measured by RT-PCR and immunohisto-chemistry respectively.Results The expression levels of HSPB7 mRNA and protein were significantly higher in the AMI group and P group than those of SH group（P＆lt;0.01）. The expression levels of HSPB7 mRNA and protein were significantly increased 1 h after AMI and reached the peak value at 3 h after AMI. The expression levels of HSPB7 mRNA and protein were still higher in 6-h group and 12-h group than those of SH group. The expression levels of HSPB7 were higher in differ-ent time points of P group than those of AMI group. Conclusion HSPB7 could express in the early stage of acute myocardi-al infarction in rats. Pravastatin could promote the upregulation of HSPB7 in myocardial infarcted border zone after AMI, which may play a protective role in early myocardial infarction.

关 键 词：热休克蛋白质类 普伐他丁 心肌梗死 急性病 疾病模型 动物 热休克蛋白B7 

分 类 号：R542.2[医药卫生—心血管疾病]