中药复方藤黄霖对微波辐照大鼠外周血T、B淋巴细胞亚群的影响  

Effects of traditional Chinese medicine Tenghuanglin on peripheral T and B lymphocyte subsets in microwave irradiated rats

作　　者：马琼[1] 杜丽[1] 崔玉芳[1] 蔡金玲[1] 杨晓云[1] 孙嵘[1] 满其航[1] 高月[1] 毛建平[1] 

机构地区：[1]北京军事医学科学院放射与辐射医学研究所,100850

出　　处：《解放军医学杂志》2014年第3期231-234,共4页Medical Journal of Chinese People's Liberation Army

基　　金：全军“十二五”医学科研重点课题(BWS11J009);毫米波国家重点实验室开放课题(K201225)~~

摘　　要：目的观察藤黄霖对微波辐照大鼠外周血T、B淋巴细胞亚群的影响,探讨其对微波诱发免疫损伤的防护作用及相关机制。方法清洁级雄性SD大鼠80只,随机分为正常对照组(CON组)、辐照对照组(RAD组)、辐照+阳性药(安多霖)对照组(ADL组)和辐照+藤黄霖组(THL组),每组20只。大鼠于辐照前7d开始灌胃给药,1次/d,连续给药7d后,用30mW/cm2微波源全身一次辐照15min。CON组行假辐照。于辐照后第7天(n=10)和第14天(n=10)活杀大鼠,检测外周血血象,CD3+、CD4+、CD8+T细胞亚群和CD45RA+B细胞亚群的变化。结果微波辐照后第14天,RAD组外周血红细胞数明显低于CON、ADL和THL组(P<0.05),而后3组间比较差异无统计学意义。辐照后第7天,RAD组外周血CD3+、CD4+、CD8+T细胞比例均明显低于CON组(P<0.05),至辐照后第14天仍未恢复到正常水平。辐照后第7天THL组和ADL组外周血CD3+、CD4+、CD8+T细胞比例均明显高于RAD组(P<0.05),至第14天CD3+、CD4+T细胞比例仍高于RAD组(P<0.05),而THL组和ADL组之间比较差异无统计学意义。辐照后第7天,RAD组外周血CD4/CD8比值明显低于CON组及THL组(P<0.05),而CON、ADL、THL组间比较差异无统计学意义。辐照后第14天,各组外周血CD4/CD8比值差异无统计学意义。辐照后第7、14天,RAD组外周血CD45RA+B细胞明显低于CON组(P<0.05),而ADL组辐照后第7天,THL组辐照后第7、14天,外周血CD45RA+B细胞明显高于RAD组(P<0.05),与CON组比较差异无统计学意义。结论微波辐照早期大鼠外周血T、B细胞减少,CD4/CD8比值下降,从而引起机体免疫功能受抑和免疫失衡,而中药藤黄霖能够提高微波辐照后大鼠外周血T、B淋巴细胞数量,改善CD4/CD8比值失衡状态,为微波辐照损伤防治药物的研究提供了新的依据。Objective To observe the effect of Tenghuanglin （THL） on peripheral T and B lymphocyte subsets in microwave irradiated rats, and explore the protective effect of THL on injury to immunity induced by microwave irradiation and its mechanism. Methods Eighty healthy male SD rats were randomly divided into 4 groups （n--20）： normal control group （CON）, radiation group （RAD）, radiation ＋ Anduolin （positive control drug） treatment group （ADL）, and radiation ＋ THL treatment group （THL）. Rats in ADL and THL groups received gavage of the individual drug once a day for 7 days, and then exposed to 30mW/cm2 microwave whole body for 15min. Rats in RAD group received same dose of microwave irradiation, and those in CON group received sham irradiation. The animals were sacrificed on 7th and 14th day after irradiation and the changes in peripheral blood smear was observed, and CD3＋, CD4＋ and CD8＋ T lymphocyte subsets, and CD45RA B lymphocyte subset were determined. Results Fourteen days after irradiation, peripheral red blood cells in RAD group was obviously lower than those in CON, ADL and THL roups, and there was no statistically significant difference of red cell count between CON, ADL and THL roups. Seven daysafter irradiation, peripheral CD3＋, CD4＋ and CD8＋ T ceils in RAD group were lower in number than those in CON group, and they did not return to normal level up to 14th day. The peripheral CD3＋, CD4＋ and CD8＋ T cells in THL and ADL groups were obviously higher than those in RAD group, and peripheral CD3＋, CD4＋ T cells were still higher than those in RAD group 14d after irradiation, while the peripheral CD3＋, CD4＋ and CD8＋ T cells in THL and ADL groups were similar both at 7th and 14th day. Seven days after irradiation, the CD4/CD8 ratio was lower in RAD group than that in CON and THL groups （P〈0.05）＋ while the ratio in CON, ADL and THL groups was similar. The CD4/CD8 ratio in 4 groups was similar on 14th day after irradiation. Peripheral CD45RA＋ B lymp

关 键 词：微波 辐照损伤 T淋巴细胞亚群 B淋巴细胞 

分 类 号：R392.12[医药卫生—免疫学]