拟南芥中缺铁反应性microRNAs的鉴定  

Identification of microRNAs Responsed to Iron Deficiency in Arabidopsis

作　　者：李华[1] 郜明泉 苏小雨[1] 赵会杰[1] 

机构地区：[1]河南农业大学生命科学学院,郑州450002

出　　处：《中国生物化学与分子生物学报》2014年第3期291-297,共7页Chinese Journal of Biochemistry and Molecular Biology

摘　　要：microRNA是一种非编码蛋白质的小分子RNA,参与了植物生长发育及环境胁迫响应的调控,主要通过对靶基因的负调控去影响生物学过程.基于前人对拟南芥全基因组microRNAs及其靶基因的预测,我们找到了靶向15个缺铁响应基因的22个microRNAs(miR158a、miR164c、miR172a、miR1887、miR2111ab、miR3933、miR395ade、miR414、miR828、miR831、miR837-3P、miR837-5P、miR854abcd、miR857、miR861-5P、miR864-5P).对这些microRNAs的启动子进行分析,发现分别有17、10和4个microRNAs启动子中包含缺铁响应元件IDE1、生长素响应元件和乙烯响应元件.进一步通过Poly(T)adaptor RT-PCR方法对这22个microRNAs在缺铁条件下的表达变化做了检测,结果显示,除miR158a和miR837-5P外的20个microRNAs在缺铁条件下的表达变化都有显著差异,且具有时间依赖性.这20个microRNAs可作为缺铁响应的候选microRNAs.microRNA （miRNA） is a non-protein-coding small molecule RNA （small RNA）, involved in plant growth and development or stress response, mainly through negative regulation of target genes to influence biological processes. Based on previous genome-wide prediction about microRNAs and their targets in Arabidopsis, we found 22 microRNAs （miR158a, miR164c, miR172a, miR1887, miR2111ab, miR3933, miR395ade, miR414, miR828, miR831, miR837-3P, miR837-SP, miR854abcd, miR857, miR861-SP, miR864-SP） targeting 15 genes related to iron deficiency. Promoter analysis of these microRNAs showed that there were 17, 10 and 4 microRNAs existing iron deficient responsive element 1 （IDE1）, auxin responsive element and ethylene responsive element, respectively. Further, Poly（T） adaptor RT-PCR was used to survey the expression of these 22 microRNAs under iron deficiency. Results showed that 20 microRNAs （except miR158a and miR837-SP） were up-regulated significantly depending on time course. In summary, these 20 microRNAs can be considered as candidate microRNAs in response to iron deficiency.

关 键 词：缺铁 MICRORNAS 拟南芥 

分 类 号：Q7[生物学—分子生物学]