异丙肾上腺素对大鼠心肌缺血再灌注损伤中高迁移率族蛋白1表达的影响及机制  

Effect of isoproterenol on expression of high mobility group box 1 protein in rats with myocardial I/R injury

作　　者：王继春[1] 胡笑容[1] 谢菁[1] 周晓亚[1] 江洪[1] 

机构地区：[1]武汉大学人民医院心内科武汉大学心血管病研究所,430060

出　　处：《中华老年心脑血管病杂志》2014年第3期302-306,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(81100146)

摘　　要：目的探讨异丙肾上腺素(ISO)对大鼠心肌缺血再灌注(I/R)损伤中高迁移率族蛋白1(HMGB-1)表达的影响及作用机制。方法健康雄性大鼠48只,随机分为假手术组(SO组)、I/R组、异丙肾上腺素预处理组(ISO-I/R组)、锌原卟啉Ⅸ(ZnPPⅨ)+ISO-I/R组(ZnPPⅨ组),每组12只。建立大鼠心肌I/R模型;检测各组大鼠的心肌梗死范围、肌酸激酶、乳酸脱氢酶(LDH)、TNF-α、白细胞介素(IL)6、超氧化物歧化酶(SOD)、丙二醛、血红素加氧酶1(HO-1)和HMGB-1表达的变化。结果与I/R组比较,ISO-I/R组心肌梗死面积明显缩小(P<0.01),LDH、肌酸激酶、TNF-α、IL-6、丙二醛、HMGB-1明显降低,SOD、HO-1明显升高(P<0.05);与ISO-I/R组比较,ZnPPⅨ组心肌梗死面明显扩大,LDH、肌酸激酶、丙二醛、TNF-α、IL-6、HMGB-1明显升高,SOD和HO-1明显降低(P<0.05)。结论 ISO能够明显诱导HO-1的表达并进一步抑制HMGB-1的释放,从而有效发挥对大鼠I/R损伤的心肌保护作用。Objective To study the expression of isoproterenol（ISO） on expression of high mobility group box 1 （HMGB-1） protein in rats with myocardial I/R injury.Methods Forty-eight healthy male SD rats were randomly divided into sham operated group,I/R group,ISO ＋ I/R group,ZnPPⅨ ＋ISO＋I/R group （12 in each group）.A rat myocardial I/R model was established.Myocardial infarction size,serum levels of creatine kinase,LDH,TNF-α,IL-6,SOD,methylglyoxal,HO-1,and expression level of HMGB-1 in different groups were measured.Results The myocardial infarction size was significantly smaller and the serum levels of creatine kinase,LDH,TNF-α,IL-6,methylglyoxal,and expression level of HMGB-1 were significantly lower while the serum levels of SOD and HO-1 were significantly higher in ISO＋I/R group han in I/R group （P〈 0.05）.The myocardial infarctin size was significantly larger and the serum levels of creatine kinase,LDH,TNF-α,IL-6,and expression level of HMGB-1 were significantly higher while the serum levels of SOD and HO-1 were significantly lower in ZnPPⅨ ＋ISO＋I/R group than in ISO＋ I/R group （P〈0.05）.Conclusion ISO plays an important role in protecting rats against myocardial I/R injury by inducing HO-1 expression and inhibiting HMGB-1 release.

关 键 词：异丙肾上腺素 心肌再灌注损伤 心肌梗死 高迁移率族蛋白质类 白细胞介素6 血红素加氧酶-1 超氧化物歧化酶 肿瘤坏死因子α 

分 类 号：R542.22[医药卫生—心血管疾病]