光谱法研究PBBHAMF与小牛胸腺DNA的相互作用  

Spectroscopic Studies on the Interaction between PBBHAMF and Calf Thymus DNA

作　　者：王瑞玲[1] 徐丽繁[1] 邓承雨[1] 黄小艳[1] 黄振钟[1] 

机构地区：[1]江西师范大学化学化工学院,江西南昌330022

出　　处：《江西师范大学学报（自然科学版）》2014年第1期6-10,共5页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：江西省教育厅2012年度科技课题(GJJ12201)资助项目

摘　　要：以小牛胸腺DNA为研究对象,探讨了4'-苯基-3-溴-8-[N,N-二(2-羟基乙基)氨基甲基]黄酮(PBBHAMF)与小牛胸腺DNA之间的相互作用模式.在以吖啶橙(AO)为荧光探针的实验中,DNA-AO复合物的荧光被PBBHAMF猝灭,其猝灭过程主要为静态猝灭;DNA的存在使PBBHAMF的紫外光谱发生了减色效应,DNA的粘度增大,CD光谱274 nm处信号发生变化,这些都能判断PBBHAMF与DNA发生了嵌插结合.此外,红外光谱结果表明PBBHAMF与DNA还存在静电结合.The interaction between 4＇-phenyl-3-bromo-8-[ N, N-bis （ 2-hydroxyethyl ） aminomethyl flavone （PBBHAMF） and calf thymus DNA （ct-DNA）has been studied. PBBHAMF could quench the fluorescence of the AO-DNA complex with the use of acridine orange（AO）as a fluorescent probe, the quenching mechanism is mainly a static quenching procedure. When bound to DNA, PBBHAMF shows hypochromism in absorption spectra, that means intercalation of PBBHAMF into DNA. Moreover, the increase in relative viscosity and the CD spectral change in 274 nm also support that the major binding mode is an inserting interaction. The FT-IR spectroscopy demonstrates that electrostatic attraction exists between PBBHAMF and DNA simultaneously.

关 键 词：4’-苯基-3-溴-8-[N N-二(2-羟基乙基)氨基甲基]黄酮 小牛胸腺DNA 荧光光谱 圆二色谱 红外光谱 

分 类 号：O657.3[理学—分析化学]