葎草花粉主要过敏原的分离鉴定与纯化  

The Characterization for the Antigenic Properties of Pollen Allergens in Humulus scandens Lour

作　　者：李莹[1] 肖小军[2] 柴文戍[1] 谢水祥[3] 何韶衡[1] 杨平常[1,2] 刘志刚[1,2] 

机构地区：[1]辽宁医学院附属第一医院,辽宁沈阳121000 [2]深圳大学医学院过敏反应与免疫学研究所,广东深圳518060 [3]赣南医学院原生物学教研室,江西赣州341000

出　　处：《江西师范大学学报（自然科学版）》2014年第1期26-29,共4页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家自然科学基金(811160129;81302553);广东省高等学校国际暨港澳台科技合作创新平台项目(2012gjhz0009);深圳市科技计划国际科技合作(GJHZ20130408174112021)资助项目

摘　　要：采用液氮研磨法提取葎草花粉粗蛋白，凝胶电泳（SDS．PAGE）分离粗提液蛋白组分并测定各组分的相对分子质量，后经过离子交换层析法分离纯化几类主要的蛋白，再用westenblotting分别检测其与花粉过敏患者血清IgE结合情况，鉴定每种主要蛋白质致敏原的致敏性．分析了葎草花粉变应原成分及其变应原性、免疫原性，并鉴定主要过敏原的致敏性．SDS-PAGE凝胶电泳结果表明，广泛的分布于8—170kDa之间有主带13条，次带10余条；westenblotting检测发现蓓草花粉的致敏性较强，与花粉过敏患者血清IgE结合能力较高．离子交换层析出4种主要变应原蛋白，其相对分子质量分别为55，16，15和25kDa，其中结合能力最强的是15kDa处蛋白条带．蓓草的主要过敏原为55，16，15和25kDa蛋白质组分．To characterize and purify the pollen allergens isolated from Humulus scandens Lour, identified the aller- genicity of these main allergen. The pollen of Humulus scandens Lour were grinded in liquid nitrogen to extract the total protin, analyzed total protin with SDS-PAGE, detective the proteins＇ allergenicity with westen-blotting, Ion-ex- change chromatography with DE-52 was used for the purification of the antigens. For the pollen extract of Humulus scandens Lour,30 protein bands were detected in SDS-PAGE. Among them,weights bewteen 8 and 170 kDa has 13 main protiens, more than 10 kinds of secondary protiens. Fore of these protein bands, with a molecular weight of 55 kDa, 16 kDa and 15 kDa,25 kDa,showed immunoreactivity with IgE in the sera from patients with allergy to pol- len of Humulus scandens Lour. Pollen allergens from Humulus scandens Lour were purified and characterized. The re- sult will provide a theoretical foundation for the diagnosis and treatment of allergy to the pollen allergens from Hu- mulus scandens Lour.

关 键 词：葎草 花粉变应原 离子交换层析 

分 类 号：R593.1[医药卫生—内科学]