卡托普利对压力负荷增加大鼠血清TNF-α表达的影响  

Effect of captopril on the expression levels of serum TNF-α in rats with pressure overload

作　　者：赵智明[1] 张静[1] 董晓蕾[1] 赵凌杰[1] 蔡辉[1] 

机构地区：[1]中国人民解放军南京军区南京总医院中西医结合科,南京市210002

出　　处：《河北医药》2014年第5期665-667,共3页Hebei Medical Journal

摘　　要：目的观察卡托普利对压力负荷增加大鼠血清TNF-α表达水平的影响。方法采用腹主动脉缩窄术制备压力负荷心肌纤维化模型,随机分成3组:假手术组、模型组和卡托普利组。术后4周成功造模并开始给药,疗程4周。8周后观察3组心脏质量指数和左心室质量指数、HE染色法观察左心室心肌病理学、Masson染色法观察左心室心肌胶原形态,图像分析测量胶原容积分数(CVF)和血管周围胶原面积(PVCA),ELISA法测定大鼠血清TNF-α的含量。结果与假手术组比较,模型组大鼠心脏质量指数和左心室质量指数均明显升高(P<0.01);与模型组比较,卡托普利组则均显著降低(P<0.01)。与假手术组比较,模型组大鼠CVF和PVCA均明显升高(P<0.01);与模型组比较,卡托普利组则均显著降低(P<0.01)。与假手术组比较,模型组大鼠血清TNF-α显著升高(P<0.01);与模型组比较,卡托普利组则显著降低(P<0.01)。结论卡托普利具有逆转心肌纤维化的作用。Objective To observe the effect of captopril on the expression levels of serum TNF-αin rats with pressure overload.Methods The animal models with pressure overload myocardial fibrosis were established by abdominal aorta constriction in Sprague-Dawley （ SD ） rats.The rats were randomly divided into three groups ：shamo-peration group , model group and captopril group .After 4 weeks, the models were successfully established and the rats were given drugs , with a treatmentcourse of 4 weeks.After 8 weeks the heart mass index and left ventricular mass index were measured ,left ventricular myocardial pathological changes were observed by means of HE staining , left ventricular myocardial collagen forms were observed by means of Masson staining ,and image analysis collagen volume fraction （ CVF）and perivascular collagen area （ PVCA） were measured by image analysis ,and the levels of serum TNF-αwere detected by ELISA .Resu lts As compared with those in sham-operation group ,the heart mass index and left ventricular mass index were significantly increased in model group （ P ＆lt;0.01）;as compared with those in model group,which in captopril group were significantly decreased （ P ＆lt;0.01）.As compared with those in sham-operation group,CVF and PVCA in model group were significantly increased （ P ＆lt;0.01）; as compared with those in model group ,which in captopril group were significantly decreased （ P ＆lt;0.01）.As compared with those in sham-operation group , the serum levels of TNF-αwere obviously increased in model group （ P ＆lt;00.1）;as compared with those in model group ,which in captopril group were significantly decreased （ P ＆lt;0.01）.Conclusion The captopril has the effect of regressing myocardial fibrosis.

关 键 词：卡托普利 压力负荷 肿瘤坏死因子-Α 大鼠 TNF-Α 

分 类 号：R285.5[医药卫生—中药学]