严重土壤干旱后复水条件下转基因甘薯的光合响应特性  

Photosynthetic characteristics of rewetted transgenic sweet potato after severe drought condition and rewetted

作　　者：陆燕元[1] 马焕成[1] 邓西平[2] 

机构地区：[1]西南林业大学林学院/西南生物多样性保育国家林业局重点实验室,云南昆明650224 [2]黄土高原土壤侵蚀与旱地农业国家重点实验室,陕西杨凌712100

出　　处：《广东农业科学》2014年第2期9-13,共5页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31260175)

摘　　要：采用盆栽试验,在重度干旱胁迫（土壤田间持水量的35％～40％）及复水条件下,对同时转入Cu/Zn SOD和APX基因的甘薯和未转基因对照植株的光合响应特性进行研究.结果表明：干旱胁迫及复水过程中转基因和未转基因植株光响应曲线的变化趋势基本一致,采用非直角双曲线模型拟合良好;转基因（TS）和未转基因甘薯（NS）的最大净光合速率（Pmax）、光饱和点（LSP）和表观量子效率（（Φ））、暗呼吸速率（Rd）在干旱胁迫下均明显降低,复水后又逐渐增加,但与NS植株相比,TS植株在胁迫条件下降低的幅度较小,而在复水过程中恢复得也更快,反映出转入的抗氧化酶基因提高了甘薯在干旱胁迫条件下的光合能力及修复能力,增强了对干旱胁迫的适应性;TS和NS植株的光补偿点（LCP）在干旱胁迫下显著提高,但TS植株上升的幅度比NS植株小,复水过程中修复得也更快,表明转基因甘薯增强了对光环境的适应性.A comparative study on photosynthetic characteristics between the genetic modified sweet potato transferred with the CU/Zn SOD and APX genes （TS） and non-GM sweet potato （NS） was carried out with pot experiments. Three controls, namely the favorable soil moisture （75%-80% of field water capacity, FWC）, the moderate drought （60%-65% of FWC） and the severe drought （35%-40% of FWC） groups were used. The Result showed that light response curves of the TS and NS had no significant difference under these three soil moisture conditions. The fitting result showed good performance by non -rectangular hyperbolic model. The net photosynthetic rate （P max）, light saturation point （LSP） and apparent quantum efficiency （φ）, dark respiration rate （Rd） of both TS and NS plants decreased significantly under drought conditions and then continually increased. However, the decrease of TS plant was less than NS plant under stress condition and the recovering was quicker. These results indicated that overexpressing antioxidant enzyme genes enhanced sweet potato photosynthetic capacity, adaptability of drought stress and recovery capability. Light compensation point （LCP） of sweet potato increased significantly under drought stress, but the rise in TS plants was less than NS control. These results showed that transgenic genes to sweet potato enhanced its light environmental adaptability.

关 键 词：转基因 甘薯 干旱 复水 光合响应曲线 

分 类 号：S531[农业科学—作物学] Q945.78[生物学—植物学]