绵毛银桦的组织培养与快速繁殖  

Tissue culture and rapid propagation of Grevillea lanigera

作　　者：龚峥[1] 张卫华[1] 张方秋[1] 王洪峰[1] 张弘[1] 何春梅[1] 徐巧林[1] 

机构地区：[1]广东省林业科学研究院,广东广州510520

出　　处：《广东农业科学》2014年第2期57-60,共4页Guangdong Agricultural Sciences

基　　金：国家林业局"948"项目(2009-4-19)

摘　　要：以枝芽为外植体,从诱导培养、增殖培养、生根培养和再生植株移栽等方面,对绵毛银桦组织培养快繁育苗技术进行试验。结果表明,使用两种浓度(0.025%和0.1%)升汞液进行二次消毒的处理方法可将外植体污染率控制在28.1%以下。适宜绵毛银桦不定芽诱导和增殖的培养基组分为WPM+6-BA 1.0 mg/L+IBA 0.2 mg/L+蔗糖30 g/L,适宜的继代培养周期为20 d,持续增殖培养的平均增殖倍数为1.65。适宜诱导生根的培养基组分为1/2WPM+IBA0.6 mg/L+蔗糖15 g/L,生根率达91.7%。移植的再生植株90%以上存活。This paper took the preliminary trial on tissue culture and rapid propagation of Grevillea lanigera. The results showed that the contamination rate of explants could be controlled below 28.1 % through the method of twice disinfection using mercuric chloride （0.025% and 0.1 %）. According to the results, the optimum medium for bud induction and multiplication was WPM＋6-BA 1.0 mg/L＋IBA 0.2 mg/Ls-sucrose 30 giL; the suitable subculture cycle was 20 d; and the proliferation multiples of adventitious buds reached 1.65. The optimum medium for root induction was 1I2WPM ＋IBA 0.6 mg/L＋sucrose 15 gIL and 91.7% of rooting percentage was obtained. The plantlet survival rate could exceed 90%.

关 键 词：绵毛银桦 组织培养 快速繁殖 

分 类 号：S722.37[农业科学—林木遗传育种]