CaCl_2处理对不同树龄库尔勒香梨提高抗寒性的研究  

Research on Improving Cold Resistance of Different Ages of Korla Fragrant Pear by CaCl_2 Treatment

作　　者：肖坤[1] 克热木.伊力 王一静[1] 买合木提.卡热 

机构地区：[1]新疆农业大学林学与园艺学院,乌鲁木齐830052

出　　处：《新疆农业科学》2014年第2期241-249,共9页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(30960234);新疆农业大学前期研究项目;库尔勒市科技项目;新疆维吾尔自治区果树学重点学科

摘　　要：【目的】研究不同浓度CaCl2处理与提高不同树龄库尔勒香梨树抗寒性的关系。【方法】以5年生、15年生、25年生库尔勒香梨树为试验材料,在展叶期(2012年5月5日)第一次喷施10、15、20、25 mmol/L浓度的CaCl2,每隔20 d喷施一次,共喷施三次,在休眠期枝条取样,在人工低温胁迫下测定膜透性和脯氨酸含量。【结果】随着低温胁迫温度的降低,不同浓度CaCl2处理后,香梨树1年生枝条的膜透性、脯氨酸的含量有不同程度的增加;5年、25年生库尔勒香梨树20 mmol/L CaCl2处理时,15年生库尔勒香梨树10 mmol/L CaCl2处理时可有效的提高膜保护能力,提高脯氨酸的含量。5年生库尔勒香梨树脯氨酸含量累积量最多,高出15年生的香梨树脯氨酸累积量的78.78%,增加幅度为5.86倍,膜透性增加幅度为3.85倍。15年生香梨树膜透性增加幅度为3.11倍,脯氨酸含量增加幅度为79.05%。25年生香梨树膜透性增加幅度为2.58倍,脯氨酸含量增加幅度为88.64%。【结论】喷施CaCl2可以提高库尔勒香梨树的抗寒性。研究是以不同生长期叶面喷施进行的,而且人工低温胁迫下测定枝条的抗寒性时离母体进行,今后不同试验方法对提高抗寒性需要进一步深入研究。[Objective] This project aims to conduct study on the relationship between CaCl2 of different concentrations and different ages to improve the cold resistance of Korla Fragrant Pears.[Method] 5a,15a and 25a of Korla Fragrant Pear trees were taken as the test materials,and during the leaf expansion period （from May 5,2012）,10 mmol/L,15 mmol/L,20 mmol/L,25 mmol/L concentrations of CaCl2 were sprayed every other 20 days,altogether three times.During the period of dormancy,branches were sampled and membrane permeability and proline content in the artificial cryogenic stress were determined.[Result] As the stress low temperature decreased,the membrane permeability,proline content of different Pear 1 a branches increased to varying degrees.Spraying CaCl2 could improve the cold resistance of Korla Fragrant Pear.5a and 25a Korla Fragrant Pear trees after 20 mmol/L CaCl2 treatment,15a Korla Fragrant Pear trees＇membrane protective ability could be improved by 10 mmol/L CaCl2 treatment effectively,then the content of proline,and the cell osmotic adjustment ability were improved,as well.The accumulated amount of proline content of the 5a Korla Fragrant Pear trees reached the maximum degree,78.78％ higher than the 15a the Fragrant Pear tree proline accumulation.The increased rate was 5.86 times,and the membrane permeability increased by 3.85 times.Membrane permeability of 15a Fragrant Pear trees was increased by 3.11 times,and the proline content increased by 79.05％.Membrane permeability of 25a Fragrant Pear trees increased by 2.58 times,and the proline content increased by 88.64％.[Conclusion] Spraying CaCl2 can improve the cold resistance of Korla Fragrant Pear trees.But the research was carried out in different growth periods of foliar spraying,and in artificial cryogenic stress,the cold resistance determination of branches was away from the parent,so in the future,different testing methods of improving cold resistance need further research.

关 键 词：CACL2处理 库尔勒香梨树 枝条 抗寒性 

分 类 号：S661.2[农业科学—果树学]