热休克蛋白70对心脏移植供心保护作用的实验研究  

Heat shock protein 70 induced by heat stress protects heterotopically transplanted hearts in rats

作　　者：刁力为[1] 刘大勇 孙振东[1] 亓峰[1] 吴乃石[1] 高宇 

机构地区：[1]哈尔滨医科大学第二临床医学院心外科,哈尔滨150086 [2]大庆市龙南医院心胸外科 [3]哈尔滨市儿童医院护理部

出　　处：《心肺血管病杂志》2014年第1期115-118,共4页Journal of Cardiovascular and Pulmonary Diseases

基　　金：黑龙江省教育厅科技研究项目资助(No.12511300)

摘　　要：目的:本实验通过热休克预适应产生热休克蛋白70,研究其是否对心脏移植中的供心有保护作用。方法:建立大鼠异位心脏移植模型并随机分为6组:对照组,经热处理的R0h、R24h、R48h、R96h及R192h实验组。分别于移植后24h取出供心,测定供心中HSP70含量,同时测定受体血液中和供心组织中心肌酶学变化并观察供心组织超微结构的变化。结果:HSP70在R24h组中表达明显增高(P<0.01),在R48h和R96h组中逐渐下降,在对照组、R0h组、R192h组表达无差异(P>0.05)。同时,R24h组受体中的乳酸脱氢酶和肌酸激酶浓度最低,供心的炎症损伤最小。结论:热休克预适应可能是一种临床中切实可行的减轻心肌缺血缺氧损伤的方法。热休克预适应-HSP70-心肌保护作用之间存在时间-剂量-疗效的关联。Objective：This study investigated whether HSP70 induced by heat stress protects heterotopically transplanted donor hearts.Methods：A total of 60 donor rats were divided randomly into six groups.Five groups received heat stress and one was a control group.Donor hearts were heterotopically transplanted into recipient rats at five time points after heat stress （0h,24h,48h,96h,192h）.The levels of HSP70 expression in donor hearts and the variation of myocardial enzymes in receptor blood or donor hearts were measured 24h after transplantation.The donated hearts were also examined microscopically for pathological changes.Results：HSP70 expression was highest in the 24h group （P ＜0.01） and decreased gradually in the 48h group and the 96h group.HSP70 expression did not differ significantly among the control group,the 0h group and the 192h group （P ＞ 0.05）.The 24h group had the lowest lactate dehydrogenase and creatine kinase muscle band concentrations in receptor blood of all the groups ; this group also showed the lowest atriphosphate and malondialdehyde concentrations （P ＜ 0.01） and mildest inflammatory injury in the transplanted hearts.Conclusion：Heat stress may be a practical way for clinical application to protect donor hearts from ischemia-reperfusion injury by inducing endogenous HSP70.We found a time-dose-effect relationship among heat stress,HSP70,and the protection of donor hearts.

关 键 词：心脏移植 热休克蛋白70 热休克预适应 供心保护 

分 类 号：R54[医药卫生—心血管疾病]