孕妇尿样中邻苯二甲酸酯代谢产物和脐带血中睾丸甾体激素水平的关联性研究  

Study on the association between maternal urinary phthalate metabolites and testicular steroid hormones in the cord blood in a Chinese population

作　　者：陈曦[1] 马静[1] 于浩[1] 冷玲[1] 周庆红[2] 孙增荣[1] 汤乃军[1] 

机构地区：[1]天津医科大学公共卫生学院劳动卫生与环境卫生学系,300070 [2]天津市疾病预防控制中心卫生检测所毒理室

出　　处：《中华预防医学杂志》2014年第3期167-171,共5页Chinese Journal of Preventive Medicine

基　　金：天津市科技支撑重点项目（09ZCGYSF01700）

摘　　要：目的 研究孕妇尿样中邻苯二甲酸酯（PAEs）代谢产物与脐带血中抑制素B（INHB）、胰岛素样因子3（INSL3）水平的关联性.方法 将2011年6月至2012年9月在天津某三级甲等妇产科医院进行产前检查的孕妇作为研究对象,采集孕期晨尿中段尿,随访其妊娠过程直至分娩,抽取脐带血10 ml,追踪到符合条件的孕妇-新生儿共69对.应用固相萃取-三重四极杆高效液相色谱串联质谱技术检测孕期尿样中PAEs的4种代谢产物[邻苯二甲酸单甲酯（MMP）、邻苯二甲酸单乙基己基酯（MEHP）、邻苯二甲酸单乙酯（MEP）、邻苯二甲酸单丁酯（MBP）]水平.应用ELISA测定脐血样品中INHB、INSL3水平,应用多元线性回归模型进行关联性分析.结果 孕妇尿中MMP、MEHP、MEP和MBP中位数分别为49.74、24.96、19.52和17.73 μg/L；脐带血中INHB和INSL3中位数分别为89.09和106.21 ng/L.MMP（β ＇=-0.252）、MEP（β＇=-0.363）和∑PAEs（β＇=-0.346）的改变对INHB水平的影响具有统计学意义,且呈负相关；∑PAEs（β＇=-0.313）的改变对INSL3水平的影响具有统计学意义,且呈负相关.结论 孕期暴露PAEs代谢产物水平与脐血中INHB和INSL3水平具有关联性.Objective The purposes of our study were to investigate the association between maternal urinary phthalate metabolites and the levels of inhibin B （INHB） and insulin-like factor 3 （INSL3）in the cord blood in a Chinese pregnant population.Methods Maternal urine samples in the third trimester of pregnancy of 69 participants were collected and stored,and the samples of cord blood（10 ml） were collected at delivery between June 2011 and September 2012 in a comprehensive hospital of gynecology and obstetrics in Tianjin,China.Four phthalate metabolites,monomethyl phthalate （MMP）,monoethyl phthalate （MEP）,monobutyl phthalate （M BP）,and mono-2-ethylhexyl phthalate （MEHP） were measured in the urine samples using liquid chromatography-tandem mass spectrometry.The levels of INHB,INSL3 in the cord blood were tested by ELISA.Associations of phthalate exposure with INHB and INSL3 levels were determined by spearman correlation and multiple regression model analysis.Results The median concentrations of observed metabolites in descending order were 49.74 μg/L for MMP,24.96 μg/L for MEHP,19.52 μg/L for MEP and 17.73 μg/L for MBP.The median concentrations of INHB and INSL3 were 89.09 and 106.21 ng/L.Significant negative associations between INHB and MMP （β＇ =-0.252）,MEP （β＇ =-0.363） or the sum value （ΣPAEs） （β＇ =-0.346） were found by the multiple regression model analysis.For INSL3,only the sum value （β＇ =-0.313） was inversely significantly associated with the levels of INSL3 in the cord blood.Conclusions Maternal urinary phthalate metabolites were associated with INHB and INSL3 in the cord blood in a Chinese population.

关 键 词：尿 胎血 抑制素类 邻苯二甲酸酯 胰岛素样因子3 

分 类 号：R446.12[医药卫生—诊断学]